ArticlePlant molecular biology2011
An improved particle bombardment for the generation of transgenic plants by direct immobilization of relleasable Tn5 transposases onto gold particles.
Article in Plant molecular biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement.Frontiers in plant science · 2021Review
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Proteolistics: a biolistic method for intracellular delivery of proteins.Transgenic research · 2014Article
- pH-dependent activation of Streptomyces hygroscopicus transglutaminase mediated by intein.Applied and environmental microbiology · 2014Article
- Genome expression profile analysis of the immature maize embryo during dedifferentiation.PloS one · 2012Article
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Authors and funding
6 authors.
Funding
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Abstract
We have developed a modified particle bombardment method for plant transgenesis. An intein-tag and a 6×Cys-tag were successively fused to the N-terminus of a hyperactive Tn5 transposase. The modified transposase was immobilized on bare gold microscopic particles via covalent binding of a 6×Cys-tag sulfydryl groups to the gold surface. The tethered transposase can bind the transposon DNA in vitro to form the transposome in the absence of Mg²⁺ ions. After bombardment of the gold particles carrying the transposomes into the plant cells, the transposomes will be released from the carrier due to the activated self-cleavage function of intein-tag. Our data showed this procedure integrated foreign DNA into the plant genome with an increased transformation frequency as compared to the conventional particle bombardment method. A single copy insertion can also be obtained by decreasing of the assembled transposon DNA amount in relation to plant cell biomass.
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