ArticlePlant cell reports2011
Variation in GUS activity in vegetatively propagated Hevea brasiliensis transgenic plants.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Optimization of somatic embryogenesis protocol for Hevea brasiliensis clones RRIM 600 and REYAN 88-13.Journal, genetic engineering & biotechnology · 2026Article
- Natural variation of the MIR168a promoter influences its activity and miR168a abundance in rice.Rice (New York, N.Y.) · 2025Article
- Micrografting Technique ofBio-protocol · 2025Article
- Optimization of the Transformation Protocol for Increased Efficiency of Genetic Transformation inPlants (Basel, Switzerland) · 2022Article
- Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees.Frontiers in plant science · 2021Review
- Genomic stability and long-term transgene expression in poplar.Transgenic research · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hevea brasiliensis transgenic plants are regenerated from transgenic callus lines by somatic embryogenesis. Somatic embryogenesis is not yet available for commercial propagation of Hevea clones, which requires conventional grafting of buds on rootstock seedlings (budding). The stability of transgene expression in budded plants is therefore necessary for further development of genetic engineering in rubber trees. Transgene expression was assessed by fluorimetric beta-glucuronidase (GUS) activity in fully developed leaves of in vitro plants from transgenic lines and their sub-lines obtained by budding. A large variation in GUS activity was found in self-rooted in vitro plants of five transgenic lines, and the absence of activity in one line suggested transgene silencing. Beyond confirming transmissibility of the reporter gene by budding and long-term expression, a quantification of GUS activity revealed that greater variability existed in budded plants compared to self-rooted mother in vitro plants for three transgenic lines. Although somatic embryogenesis provided more stable GUS activity, budding remained an efficient way of propagating transgenic plants but transgene expression in budded plants should be verified for functional analysis and further development.
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Registered trials
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