ArticleJournal of industrial microbiology & biotechnology2011
Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.
Article in Journal of industrial microbiology & biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- Stress-Driven Production of γ-Aminobutyric Acid Using Non-Conventional Yeast StrainsJournal of fungi (Basel, Switzerland) · 2024Article
- Lignocellulosic xylitol production from corncob using engineeredFrontiers in bioengineering and biotechnology · 2022Article
- Deletion ofFrontiers in microbiology · 2022Article
- Establishment ofJournal of fungi (Basel, Switzerland) · 2021Article
- The Model SystemLife (Basel, Switzerland) · 2020Review
- Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate.Microbial cell factories · 2019Article
- Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1-2.BMC microbiology · 2018Article
- Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism.Journal of industrial microbiology & biotechnology · 2017Article
- Article
- Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses.Biotechnology for biofuels · 2015Article
- Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain.Microbial cell factories · 2014Article
- Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway.Journal of industrial microbiology & biotechnology · 2013Article
- Improving ethanol and xylitol fermentation at elevated temperature through substitution of xylose reductase in Kluyveromyces marxianus.Journal of industrial microbiology & biotechnology · 2013Article
- Identification of a xylitol dehydrogenase gene from Kluyveromyces marxianus NBRC1777.Molecular biotechnology · 2013Article
- Genome sequence of the thermotolerant yeast Kluyveromyces marxianus var. marxianus KCTC 17555.Eukaryotic cell · 2012Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kluyveromyces marxianus is thermotolerant yeast that is able to utilize a wider range of substrates and has greater thermal tolerance than most other yeast species. K. marxianus can assimilate xylose, but its ability to produce ethanol from xylose in oxygen-limited environments is poor. In the present study, the K. marxianus xylose reductase (KmXR) gene (Kmxyl1) was cloned and the recombinant enzyme was characterized to clarify the factors that limit xylose fermentation in K. marxianus NBRC1777. KmXR is a key enzyme in the xylose metabolism of K. marxianus, which was verified by disruption of the Kmxyl1 gene. The Km of the recombinant KmXR for NADPH is 65.67 μM and KmXR activity is 1.295 U/mg, which is lower than those of most reported yeast XRs, and the enzyme has no activity with coenzyme NADH. This result demonstrates that the XR from K. marxianus is highly coenzyme specific; combined with the extremely low XDH activity of K. marxianus with NADP+, the limitation of xylose fermentation is due to a redox imbalance under anaerobic conditions and low KmXR activity.
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