ArticleJournal of bioenergetics and biomembranes2011
Online monitoring of metabolism and morphology of peptide-treated neuroblastoma cancer cells and keratinocytes.
Article in Journal of bioenergetics and biomembranes, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- K092A and K092B, Two Peptides Isolated from the Dogfish (Marine drugs · 2019Article
- Biological Activity Of miRNA-27a Using Peptide-based Drug Delivery Systems.International journal of nanomedicine · 2019Article
- Melittin, a major peptide component of bee venom, and its conjugates in cancer therapy.Cancer letters · 2017Review
- Host defense peptides for treatment of colorectal carcinoma - a comparative in vitro and in vivo analysis.Oncotarget · 2014Article
- Melittin modulates keratinocyte function through P2 receptor-dependent ADAM activation.The Journal of biological chemistry · 2012Article
- Membrane-active host defense peptides--challenges and perspectives for the development of novel anticancer drugs.Chemistry and physics of lipids · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antimicrobial peptides are promising anti-cancer agents with a unique mode of action. We established the usage of a chip-based sensor to monitor the dynamic interplay between cells on the chip and peptides and compared it with endpoint tests. Human neuroblastoma cancer cells and spontaneously immortalized non-cancer keratinocytes were perfused with representative peptides (NK-2, NK11, and melittin). The sensor system enabled continuous recording of cell layer impedance (adhesion/confluence), oxygen consumption (respiration) and extracellular acidification (glycolysis) and provided insights in cell damage, stress response and recovery. Cells responded differentially to peptide treatment. During perfusion, peptides accumulated on the cell surface until they reached a critical concentration. Preceding to cell death, melittin triggered glycolysis, suggesting stress response. NK-2 induced no change in energy metabolism, but led to an increase in impedance, i.e. a temporarily altered morphology, which appeared to be an excellent parameter to detect subtle structural changes of cell layers.
Indexed as
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.