ArticleCurrent chemical genomics2011
Two High Throughput Screen Assays for Measurement of TNF-α in THP-1 Cells.
Article in Current chemical genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
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- Systematic Identification of Molecular Targets and Pathways Related to Human Organ Level Toxicity.Chemical research in toxicology · 2021Article
- Electrochemical ELISA Protein Biosensing in Undiluted Serum Using a Polypyrrole-Based Platform.Sensors (Basel, Switzerland) · 2020Article
- Targeting hormone refractory prostate cancer by in vivo selected DNA libraries in an orthotopic xenograft mouse model.Scientific reports · 2019Article
- Expanding biological space coverage enhances the prediction of drug adverse effects in human using in vitro activity profiles.Scientific reports · 2018Article
- High Sensitivity Determination of TNF-α for Early Diagnosis of Neonatal Infections with a Novel and Reusable Electrochemical Sensor.Sensors (Basel, Switzerland) · 2017Article
- AlphaScreen-Based Assays: Ultra-High-Throughput Screening for Small-Molecule Inhibitors of Challenging Enzymes and Protein-Protein Interactions.Methods in molecular biology (Clifton, N.J.) · 2016Article
- SAR Studies of Exosite-Binding Substrate-Selective Inhibitors of A Disintegrin And Metalloprotease 17 (ADAM17) and Application as Selective in Vitro Probes.Journal of medicinal chemistry · 2015Article
- ERK and β-arrestin interaction: a converging point of signaling pathways for multiple types of cell surface receptors.Journal of biomolecular screening · 2015Article
- Surface-micromachined microfiltration membranes for efficient isolation and functional immunophenotyping of subpopulations of immune cells.Advanced healthcare materials · 2013Article
- An integrated microfluidic platform for in situ cellular cytokine secretion immunophenotyping.Lab on a chip · 2012Article
- Paradigm shift in toxicity testing and modeling.The AAPS journal · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Tumor Necrosis Factor-α (TNF-α), a secreted cytokine, plays an important role in inflammatory diseases and immune disorders, and is a potential target for drug development. The traditional assays for detecting TNF-α, enzyme linked immunosorbent assay (ELISA) and radioimmunoassay, are not suitable for the large size compound screens. Both assays suffer from a complicated protocol, multiple plate wash steps and/or excessive radioactive waste. A simple and quick measurement of TNF-α production in a cell based assay is needed for high throughput screening to identify the lead compounds from the compound library. We have developed and optimized two homogeneous TNF-α assays using the HTRF (homogeneous time resolved fluorescence) and AlphaLISA assay formats. We have validated the HTRF based TNF-α assay in a 1536-well plate format by screening a library of 1280 pharmacologically active compounds. The active compounds identified from the screen were confirmed in the AlphaLISA TNF-α assay using a bead-based technology. These compounds were also confirmed in a traditional ELISA assay. From this study, several beta adrenergic agonists have been identified as TNF-α inhibitors. We also identified several novel inhibitors of TNF-α, such as BTO-1, CCG-2046, ellipticine, and PD 169316. The results demonstrated that both homogeneous TNF-α assays are robust and suitable for high throughput screening.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.