ArticleInvestigative ophthalmology & visual science2011
Homocysteine-mediated modulation of mitochondrial dynamics in retinal ganglion cells.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- Can Persistent Blue Light Exposure Cause a Molecular Shift in the Vitreous of Rodents?Scientifica · 2026Article
- Hyperhomocysteinemia Induced Mitochondrial Dysfunction Disrupting the Eye Development.Neurotoxicity research · 2025Article
- Role of Tau Protein Hyperphosphorylation in Diabetic Retinal Neurodegeneration.Journal of ophthalmology · 2025Review
- Homocysteine and mitochondrial quality control in diabetic retinopathy.Eye and vision (London, England) · 2024Article
- Homocysteine and Glaucoma.International journal of molecular sciences · 2023Review
- Targeting Astrocyte Signaling Alleviates Cerebrovascular and Synaptic Function Deficits in a Diet-Based Mouse Model of Small Cerebral Vessel Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Warburg Effect as a Novel Mechanism for Homocysteine-Induced Features of Age-Related Macular Degeneration.International journal of molecular sciences · 2023Article
- Mitochondrial Fragmentation in a High Homocysteine Environment in Diabetic Retinopathy.Antioxidants (Basel, Switzerland) · 2022Article
- Effect of long-term chronic hyperhomocysteinemia on retinal structure and function in the cystathionine-β-synthase mutant mouse.Experimental eye research · 2022Article
- The effects of vitamin B12 deficiency on retina and optic disk vascular density.International ophthalmology · 2021Article
- Implication ofInternational journal of molecular sciences · 2021Article
- Homocysteine and Mitochondria in Cardiovascular and Cerebrovascular Systems.International journal of molecular sciences · 2020Review
- Diabetic Retinopathy: Mitochondria Caught in a Muddle of Homocysteine.Journal of clinical medicine · 2020Review
- The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.The Journal of biological chemistry · 2020Article
- Dietary Intakes of Vegetable Protein, Folate, and Vitamins B-6 and B-12 Are Partially Correlated with Physical Functioning of Dutch Older Adults Using Copula Graphical Models.The Journal of nutrition · 2020Article
- Faulty homocysteine recycling in diabetic retinopathy.Eye and vision (London, England) · 2020Article
- Hyperhomocysteinemia-induced death of retinal ganglion cells: The role of Müller glial cells and NRF2.Redox biology · 2019Article
- Uncoupling Protein 2 Increases Blood Pressure in DJ -1 Knockout Mice.Journal of the American Heart Association · 2019Article
- Excess homocysteine upregulates the NRF2-antioxidant pathway in retinal Müller glial cells.Experimental eye research · 2019Article
- Biothiols and oxidative stress markers and polymorphisms ofOncotarget · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo evaluate the effect of excess homocysteine on the regulation of retinal ganglion cell mitochondrial dynamics.
methodsMice deficient in cystathionine-β-synthase (cbs) were used as a model of hyperhomocysteinemia. Gene and protein expression analyses of Opa1 and Fis1 were performed on cbs⁺/⁻ neural retinas. Mitochondria within retinal ganglion cell axons underwent systematic ultrastructural analysis to measure area, length, width, and the distance between the mitochondria and the axon wall. Primary mouse ganglion cells were cultured, treated with homocysteine, and assessed for levels of Opa1 and Fis1 protein, the number of mitochondria per length of neurite, and levels of cleaved caspase-3.
resultsOpa1 and Fis1 protein levels in cbs⁺/⁻ neural retinas were elevated to 191.00% ± 26.40% and 226.20% ± 4.57%, respectively, compared with wild-type. Mitochondria of cbs⁺/⁻ retinas were smaller in all parameters studied, including area (0.32 ± 0.01 μm² vs. 0.42 ± 0.02 μm²), compared with wild-type. Primary ganglion cells treated with homocysteine had elevations in Opa1 and Fis1 proteins, a significantly higher number of mitochondria per length of neurite (0.1781 ± 0.017 vs. 0.1156 ± 0.012), and significantly higher levels of cleaved caspase-3 compared with control.
conclusionsThis study provides the first evidence that homocysteine-induced ganglion cell loss involves the dysregulation of mitochondrial dynamics, both in vivo and in vitro. The present data suggest increased mitochondrial fission as a novel mechanism of homocysteine toxicity to neurons. Of particular relevance are glaucoma and Alzheimer's disease, neurodegenerative diseases that are associated with hyperhomocysteinemia and, more recently, have implicated increased mitochondrial fission in their pathogeneses.
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