ArticleJournal of bacteriology2011
Structure-guided investigation of lipopolysaccharide O-antigen chain length regulators reveals regions critical for modal length control.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Expanded prevalence of group 4 capsule expression and its genetic requirements in a broad array ofmSphere · 2026Article
- Chain-Length Regulation by WzzE Is Necessary for, but Genetically Separable From, Cyclic Enterobacterial Common Antigen Synthesis.Molecular microbiology · 2026Article
- Structural Basis of Lipopolysaccharide O-Antigen Chain Length Modality.Research (Washington, D.C.) · 2026Article
- Genomic Markers Distinguishing Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2025Article
- Unrecognized risk of perfluorooctane sulfonate in promoting conjugative transfers of bacterial antibiotic resistance genes.Applied and environmental microbiology · 2023Article
- Alternating L4 loop architecture of the bacterial polysaccharide co-polymerase WzzE.Communications biology · 2023Article
- The effect of O-antigen length determinant wzz on the immunogenicity of Salmonella Typhimurium for Escherichia coli O2 O-polysaccharides delivery.Veterinary research · 2023Article
- Impact and Control of Sugar Size in Glycoconjugate Vaccines.Molecules (Basel, Switzerland) · 2022Review
- Combined effects of glycan chain length and linkage type on the immunogenicity of glycoconjugate vaccines.NPJ vaccines · 2021Review
- Structure of a full-length bacterial polysaccharide co-polymerase.Nature communications · 2021Article
- Lipopolysaccharide O-antigens-bacterial glycans made to measure.The Journal of biological chemistry · 2020Review
- Loss of wbpL disrupts O-polysaccharide synthesis and impairs virulence of plant-associated Pseudomonas strains.Molecular plant pathology · 2019Article
- Unique Regions of the Polysaccharide Copolymerase WzzJournal of bacteriology · 2019Article
- A lipid gating mechanism for the channel-forming O antigen ABC transporter.Nature communications · 2019Article
- Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells.PLoS pathogens · 2017Article
- Article
- The D3 bacteriophage α-polymerase inhibitor (Iap) peptide disrupts O-antigen biosynthesis through mimicry of the chain length regulator Wzz in Pseudomonas aeruginosa.Journal of bacteriology · 2013Article
- Identification of an O-antigen chain length regulator, WzzP, in Porphyromonas gingivalis.MicrobiologyOpen · 2013Article
- Combining quantitative genetic footprinting and trait enrichment analysis to identify fitness determinants of a bacterial pathogen.PLoS genetics · 2013Article
- Structural characterization of closely related O-antigen lipopolysaccharide (LPS) chain length regulators.The Journal of biological chemistry · 2012Article
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Authors and funding
4 authors.
Funding
Abstract
The O-antigen component of the lipopolysaccharide (LPS) represents a population of polysaccharide molecules with nonrandom (modal) chain length distribution. The number of the repeat O units in each individual O-antigen polymer depends on the Wzz chain length regulator, an inner membrane protein belonging to the polysaccharide copolymerase (PCP) family. Different Wzz proteins confer vastly different ranges of modal lengths (4 to >100 repeat units), despite having remarkably conserved structural folds. The molecular mechanism responsible for the selective preference for a certain number of O units is unknown. Guided by the three-dimensional structures of PCPs, we constructed a panel of chimeric molecules containing parts of two closely related Wzz proteins from Salmonella enterica and Shigella flexneri which confer different O-antigen chain length distributions. Analysis of the O-antigen length distribution imparted by each chimera revealed the region spanning amino acids 67 to 95 (region 67 to 95), region 200 to 255, and region 269 to 274 as primarily affecting the length distribution. We also showed that there is no synergy between these regions. In particular, region 269 to 274 also influenced chain length distribution mediated by two distantly related PCPs, WzzB and FepE. Furthermore, from the 3 regions uncovered in this study, region 269 to 274 appeared to be critical for the stability of the oligomeric form of Wzz, as determined by cross-linking experiments. Together, our data suggest that chain length determination depends on regions that likely contribute to stabilize a supramolecular complex.
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