ArticleApplied and environmental microbiology2011
Redesigning Escherichia coli metabolism for anaerobic production of isobutanol.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Linking genome content to biofuel production yields: a meta-analysis of major catabolic pathways among select H2 and ethanol-producing bacteria.BMC microbiology · 2012Pooled it
- Recent advances in biomass deconstruction, microbial conversion, artificial intelligence, and carbon capture for sustainable bioenergy.Bioresources and bioprocessing · 2026Review
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- Merging the computational design of chimeric type I polyketide synthases with enzymatic pathways for chemical biosynthesis.Nature communications · 2025Article
- Addressing genome scale design tradeoffs in Pseudomonas putida for bioconversion of an aromatic carbon source.NPJ systems biology and applications · 2025Article
- Engineering new-to-nature biochemical conversions by combining fermentative metabolism with respiratory modules.Nature communications · 2024Article
- Growth-coupled anaerobic production of isobutanol from glucose in minimal medium with Escherichia coli.Biotechnology for biofuels and bioproducts · 2023Article
- PyMiner: A method for metabolic pathway design based on the uniform similarity of substrate-product pairs and conditional search.PloS one · 2022Article
- Metabolic engineering of Escherichia coli for the production of isobutanol: a review.World journal of microbiology & biotechnology · 2021Review
- Isobutene production inMetabolic engineering communications · 2021Article
- Analysis of metabolic network disruption in engineered microbial hosts due to enzyme promiscuity.Metabolic engineering communications · 2021Article
- Modulating redox metabolism to improve isobutanol production in Shimwellia blattae.Biotechnology for biofuels · 2021Article
- Metabolic engineering ofBiotechnology for biofuels · 2020Article
- Evolutionary Changes after Translational Challenges Imposed by Horizontal Gene Transfer.Genome biology and evolution · 2019Article
- Microbial biosynthesis of lactate esters.Biotechnology for biofuels · 2019Article
- Article
- Harnessing a P450 fatty acid decarboxylase fromMetabolic engineering communications · 2018Article
- Review
- Enhancement of photosynthetic isobutanol production in engineered cells ofBiotechnology for biofuels · 2018Article
- Isobutanol production inMetabolic engineering communications · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fermentation enables the production of reduced metabolites, such as the biofuels ethanol and butanol, from fermentable sugars. This work demonstrates a general approach for designing and constructing a production host that uses a heterologous pathway as an obligately fermentative pathway to produce reduced metabolites, specifically, the biofuel isobutanol. Elementary mode analysis was applied to design an Escherichia coli strain optimized for isobutanol production under strictly anaerobic conditions. The central metabolism of E. coli was decomposed into 38,219 functional, unique, and elementary modes (EMs). The model predictions revealed that during anaerobic growth E. coli cannot produce isobutanol as the sole fermentative product. By deleting 7 chromosomal genes, the total 38,219 EMs were constrained to 12 EMs, 6 of which can produce high yields of isobutanol in a range from 0.29 to 0.41 g isobutanol/g glucose under anaerobic conditions. The remaining 6 EMs rely primarily on the pyruvate dehydrogenase enzyme complex (PDHC) and are typically inhibited under anaerobic conditions. The redesigned E. coli strain was constrained to employ the anaerobic isobutanol pathways through deletion of 7 chromosomal genes, addition of 2 heterologous genes, and overexpression of 5 genes. Here we present the design, construction, and characterization of an isobutanol-producing E. coli strain to illustrate the approach. The model predictions are evaluated in relation to experimental data and strategies proposed to improve anaerobic isobutanol production. We also show that the endogenous alcohol/aldehyde dehydrogenase AdhE is the key enzyme responsible for the production of isobutanol and ethanol under anaerobic conditions. The glycolytic flux can be controlled to regulate the ratio of isobutanol to ethanol production.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.