ArticleApplied and environmental microbiology2011
Decay of bacterial pathogens, fecal indicators, and real-time quantitative PCR genetic markers in manure-amended soils.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 117 citations in OpenAlex.
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- Formation of Escherichia coli O157:H7 Persister Cells in the Lettuce Phyllosphere and Application of Differential Equation Models To Predict Their Prevalence on Lettuce Plants in the Field.Applied and environmental microbiology · 2020Article
- Phenotypic variations in persistence and infectivity between and within environmentally transmitted pathogen populations impact population-level epidemic dynamics.BMC infectious diseases · 2019Article
- Removal of fecal indicator bacteria and antibiotic resistant genes in constructed wetlands.Environmental science and pollution research international · 2019Article
- Extended persistence of general and cattle-associated fecal indicators in marine and freshwater environment.The Science of the total environment · 2019Article
- Evaluating the long-term persistence of Bacillus spores on common surfaces.Microbial biotechnology · 2018Article
- Fecal Fingerprints of Enteric Pathogen Contamination in Public Environments of Kisumu, Kenya, Associated with Human Sanitation Conditions and Domestic Animals.Environmental science & technology · 2018Article
- Quantification of Leptospira interrogans Survival in Soil and Water Microcosms.Applied and environmental microbiology · 2018Article
- Antibiotic-Resistant Genes and Pathogens Shed by Wild Deer Correlate with Land Application of Residuals.EcoHealth · 2018Article
- The effect of anaerobic digestion and storage on indicator microorganisms in swine and dairy manure.Environmental science and pollution research international · 2017Article
- Sources and persistence of fecal indicator bacteria andWater, air, and soil pollution · 2017Article
- Modeling Biphasic Environmental Decay of Pathogens and Implications for Risk Analysis.Environmental science & technology · 2017Article
- Evaluation of five microbial and four mitochondrial DNA markers for tracking human and pig fecal pollution in freshwater.Scientific reports · 2016Article
- Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.Environmental science and pollution research international · 2016Article
- Depth-Dependent Survival of Escherichia coli and Enterococci in Soil after Manure Application and Simulated Rainfall.Applied and environmental microbiology · 2015Article
- Temporal stability of the microbial community in sewage-polluted seawater exposed to natural sunlight cycles and marine microbiota.Applied and environmental microbiology · 2015Article
- Evidence for extraintestinal growth of bacteroidales originating from poultry litter.Applied and environmental microbiology · 2015Article
- Hierarchal clustering yields insight into multidrug-resistant bacteria isolated from a cattle feedlot wastewater treatment system.Environmental monitoring and assessment · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study examined persistence and decay of bacterial pathogens, fecal indicator bacteria (FIB), and emerging real-time quantitative PCR (qPCR) genetic markers for rapid detection of fecal pollution in manure-amended agricultural soils. Known concentrations of transformed green fluorescent protein-expressing Escherichia coli O157:H7/pZs and red fluorescent protein-expressing Salmonella enterica serovar Typhimurium/pDs were added to laboratory-scale manure-amended soil microcosms with moisture contents of 60% or 80% field capacity and incubated at temperatures of -20°C, 10°C, or 25°C for 120 days. A two-stage first-order decay model was used to determine stage 1 and stage 2 first-order decay rate coefficients and transition times for each organism and qPCR genetic marker in each treatment. Genetic markers for FIB (Enterococcus spp., E. coli, and Bacteroidales) exhibited decay rate coefficients similar to that of E. coli O157:H7/pZs but not of S. enterica serovar Typhimurium/pDs and persisted at detectable levels longer than both pathogens. Concentrations of these two bacterial pathogens, their counterpart qPCR genetic markers (stx1 and ttrRSBCA, respectively), and FIB genetic markers were also correlated (r = 0.528 to 0.745). This suggests that these qPCR genetic markers may be reliable conservative surrogates for monitoring fecal pollution from manure-amended land. Host-associated qPCR genetic markers for microbial source tracking decayed rapidly to nondetectable concentrations, long before FIB, Salmonella enterica serovar Typhimurium/pDs, and E. coli O157:H7/pZs. Although good indicators of point source or recent nonpoint source fecal contamination events, these host-associated qPCR genetic markers may not be reliable indicators of nonpoint source fecal contamination events that occur weeks following manure application on land.
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