ArticleJournal of experimental botany2011
Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis.
Article in Journal of experimental botany, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
- An active RNA transport mechanism into plant vacuoles.Nucleic acids research · 2026Article
- Cytokinin availability and chloroplast functional lifetime in senescing leaves.Frontiers in plant science · 2026Review
- Identification of Auxin, Cytokinin, Transcription Factors, and Other Zygotic Embryogenesis-Related Genes inPlants (Basel, Switzerland) · 2025Article
- Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.Molecular horticulture · 2025Review
- Characterization and Transcriptome Analysis Reveal Abnormal Pollen Germination in Cytoplasmic Male Sterile Tomato.International journal of molecular sciences · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- SOS1 tonoplast neo-localization and the RGG protein SALTY are important in the extreme salinity tolerance of Salicornia bigelovii.Nature communications · 2024Article
- Plant Hormonomics: A Key Tool for Deep Physiological Phenotyping to Improve Crop Productivity.Plant & cell physiology · 2023Review
- A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.The Plant cell · 2023Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- 2',3'-cAMP treatment mimics the stress molecular response in Arabidopsis thaliana.Plant physiology · 2022Article
- Phosphate-deprivation and damage signalling by extracellular ATP.Frontiers in plant science · 2022Article
- Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes inInternational journal of molecular sciences · 2021Article
- Differential Subcellular Distribution of Cytokinins: How Does Membrane Transport Fit into the Big Picture?International journal of molecular sciences · 2021Review
- A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate Accumulation.The Plant cell · 2020Article
- Nucleotide Metabolism in Plants.Plant physiology · 2020Article
- Review
- Identification of microRNAs and relative target genes in Moringa oleifera leaf and callus.Scientific reports · 2019Article
- Genome-wide analysis of alternative splicing divergences between Brassica hexaploid and its parents.Planta · 2019Article
- 3D Imaging of Tapetal Mitochondria Suggests the Importance of Mitochondrial Fission in Pollen Growth.Plant physiology · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ENT1 of Arabidopsis thaliana was the first member of the equilibrative nucleoside transporter (ENT) family to be identified in plants and characterized as a cellular, high-affinity nucleoside importer. Evidence is presented here for a tonoplast localization of ENT1 based on proteome data and Western blot analyses. Increased export of adenosine from reconstituted tonoplast preparations from 35S:ENT1 mutants compared with those from the wild type and ENT1-RNAi mutants support this view. Furthermore, increased vacuolar adenosine and vacuolar 2'3'-cAMP (an intermediate of RNA catabolism) contents in ENT1-RNAi mutants, but decreased contents of these metabolites in 35S:ENT1 over-expresser mutants, were observed. An up-regulation of the salvage pathway was detected in the latter mutants, leading to the conclusion that draining the vacuolar adenosine storage by ENT1 over-expression interferes with cellular nucleotide metabolism. As a consequence of the observed metabolic alterations 35S:ENT1 over-expresser mutants exhibited a smaller phenotypic appearance compared with wild-type plants. In addition, ENT1:RNAi mutants exhibited significantly lower in vitro germination of pollen and contained reduced internal and external ATP levels. This indicates that ENT1-mediated nucleosides, especially adenosine transport, is important for nucleotide metabolism, thus influencing growth and pollen germination.
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