ArticleBiophysical journal2011
Spot variation fluorescence correlation spectroscopy allows for superresolution chronoscopy of confinement times in membranes.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.Biomolecules · 2026Article
- Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.Biomolecules · 2025Article
- Boxcar Imaging FCS Reveals Membrane Raft Stabilization Kinetics in Antigen-Stimulated Mast Cells.bioRxiv : the preprint server for biology · 2025Article
- MNature communications · 2024Article
- Single-photon microscopy to study biomolecular condensates.Nature communications · 2023Article
- The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy.Nature communications · 2022Article
- The ganglioside GM1a functions as a coreceptor/attachment factor for dengue virus during infection.The Journal of biological chemistry · 2022Article
- Computational Proposal for Tracking Multiple Molecules in a Multifocus Confocal Setup.ACS photonics · 2022Article
- Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.Plant physiology · 2022Review
- Advances in the Methods for the Synthesis of Carbon Dots and Their Emerging Applications.Polymers · 2021Review
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Increased Confinement and Polydispersity of STIM1 and Orai1 after CaBiophysical journal · 2020Article
- Do ABC transporters regulate plasma membrane organization?Cellular & molecular biology letters · 2020Review
- An alternative framework for fluorescence correlation spectroscopy.Nature communications · 2019Article
- Anomalous Diffusion in Inverted Variable-Lengthscale Fluorescence Correlation Spectroscopy.Biophysical journal · 2019Article
- Nanoscale Spatiotemporal Diffusion Modes Measured by Simultaneous Confocal and Stimulated Emission Depletion Nanoscopy Imaging.Nano letters · 2018Article
- FSCS Reveals the Complexity of Lipid Domain Dynamics in the Plasma Membrane of Live Cells.Biophysical journal · 2018Article
- Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS.Molecular biology of the cell · 2017Article
- Spot size variation FCS in simulations of the 2D Ising model.Journal of physics D: Applied physics · 2016Article
- Current approaches to studying membrane organization.F1000Research · 2015Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Resolving the dynamical interplay of proteins and lipids in the live-cell plasma membrane represents a central goal in current cell biology. Superresolution concepts have introduced a means of capturing spatial heterogeneity at a nanoscopic length scale. Similar concepts for detecting dynamical transitions (superresolution chronoscopy) are still lacking. Here, we show that recently introduced spot-variation fluorescence correlation spectroscopy allows for sensing transient confinement times of membrane constituents at dramatically improved resolution. Using standard diffraction-limited optics, spot-variation fluorescence correlation spectroscopy captures signatures of single retardation events far below the transit time of the tracer through the focal spot. We provide an analytical description of special cases of transient binding of a tracer to pointlike traps, or association of a tracer with nanodomains. The influence of trap mobility and the underlying binding kinetics are quantified. Experimental approaches are suggested that allow for gaining quantitative mechanistic insights into the interaction processes of membrane constituents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.