ArticleBiophysical journal2011
Measuring the folding landscape of a harmonically constrained biopolymer.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- -1 Programmed Ribosomal Frameshifting as a Force-Dependent Process.Progress in molecular biology and translational science · 2016Review
- Reconstructing folding energy landscapes from splitting probability analysis of single-molecule trajectories.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Single-molecule measurements of the CCR5 mRNA unfolding pathways.Biophysical journal · 2014Article
- Reconstructing folding energy landscapes by single-molecule force spectroscopy.Annual review of biophysics · 2014Review
- From mechanical folding trajectories to intrinsic energy landscapes of biopolymers.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- RNA under tension: Folding Landscapes, Kinetic partitioning Mechanism, and Molecular Tensegrity.The journal of physical chemistry letters · 2012Article
- Limitations of constant-force-feedback experiments.Biophysical journal · 2012Article
- Practical axial optical trapping.The Review of scientific instruments · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pioneering studies have shown that the probability distribution of opening length for a DNA hairpin, recorded under constant force using an optical trap, can be used to reconstruct the energy landscape of the transition. However, measurements made under constant force are subject to some limitations. Under constant force a system with a sufficiently high energy barrier spends most of its time in the closed or open conformation, with relatively few statistics collected in the transition state region. We describe a measurement scheme in which the system is driven progressively through the transition by an optical trap and an algorithm is used to extract the energy landscape of the transition from the fluctuations recorded during this process. We illustrate this technique in simulations and demonstrate its effectiveness in experiments on a DNA hairpin. We find that the combination of this technique with the use of short DNA handles facilitates a high-resolution measurement of the hairpin's folding landscape with a very short measurement time.
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