ArticleBiophysical journal2011
Reconstitution of contractile actomyosin bundles.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 139 citations in OpenAlex.
- Contractile to extensile transitions and mechanical adaptability enabled by activity in cytoskeletal structures.bioRxiv : the preprint server for biology · 2026Article
- State-dependent energy conversion produces degenerate dissipation in active actomyosin networks.bioRxiv : the preprint server for biology · 2026Article
- Rethinking contractility in active cytoskeletal matter.Biophysical journal · 2026Article
- Theory of active self-organization of dense nematic structures in the actin cytoskeleton.eLife · 2025Article
- Engineering filamentous myosins for optical control of contractility.bioRxiv : the preprint server for biology · 2025Article
- Cracked actin filaments as mechanosensitive receptors.Biophysical journal · 2024Article
- Structure and dynamics of motor-driven microtubule bundles.Soft matter · 2024Article
- Limiting pool and actin architecture controls myosin cluster sizes in adherent cells.Biophysical journal · 2024Article
- Staphylococcal Enterotoxin C2 Mutant-Induced Antitumor Immune Response Is Controlled by CDC42/MLC2-Mediated Tumor Cell Stiffness.International journal of molecular sciences · 2023Article
- Myosin and [Formula: see text]-actinin regulation of stress fiber contractility under tensile stress.Scientific reports · 2023Article
- Motor processivity and speed determine structure and dynamics of microtubule-motor assemblies.eLife · 2023Article
- Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics.Journal of visualized experiments : JoVE · 2022Article
- Article
- A generalized Flory-Stockmayer kinetic theory of connectivity percolation and rigidity percolation of cytoskeletal networks.PLoS computational biology · 2022Article
- Protein friction and filament bending facilitate contraction of disordered actomyosin networks.Biophysical journal · 2021Article
- Ventral stress fibers induce plasma membrane deformation in human fibroblasts.Molecular biology of the cell · 2021Article
- The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces.Molecular biology of the cell · 2021Article
- Bond Type and Discretization of Nonmuscle Myosin II Are Critical for Simulated Contractile Dynamics.Biophysical journal · 2020Article
- Article
- The Actin Cytoskeleton as an Active Adaptive Material.Annual review of condensed matter physics · 2020Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Contractile actomyosin bundles are critical for numerous aspects of muscle and nonmuscle cell physiology. Due to the varying composition and structure of actomyosin bundles in vivo, the minimal requirements for their contraction remain unclear. Here, we demonstrate that actin filaments and filaments of smooth muscle myosin motors can self-assemble into bundles with contractile elements that efficiently transmit actomyosin forces to cellular length scales. The contractile and force-generating potential of these minimal actomyosin bundles is sharply sensitive to the myosin density. Above a critical myosin density, these bundles are contractile and generate large tensile forces. Below this threshold, insufficient cross-linking of F-actin by myosin thick filaments prevents efficient force transmission and can result in rapid bundle disintegration. For contractile bundles, the rate of contraction decreases as forces build and stalls under loads of ∼0.5 nN. The dependence of contraction speed and stall force on bundle length is consistent with bundle contraction occurring by several contractile elements connected in series. Thus, contraction in reconstituted actomyosin bundles captures essential biophysical characteristics of myofibrils while lacking numerous molecular constituents and structural signatures of sarcomeres. These results provide insight into nonsarcomeric mechanisms of actomyosin contraction found in smooth muscle and nonmuscle cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.