ArticleThe Journal of cell biology1990
Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 98 citations in OpenAlex.
- Article
- Gelsolin-Cu/ZnSOD interaction alters intracellular reactive oxygen species levels to promote cancer cell invasion.Oncotarget · 2016Article
- DNA methyltransferase inhibitor, zebularine, delays tumor growth and induces apoptosis in a genetically engineered mouse model of breast cancer.Molecular cancer therapeutics · 2012Article
- Profilin-1 is a negative regulator of mammary carcinoma aggressiveness.British journal of cancer · 2007Article
- Cytoskeletal rearrangements in synovial fibroblasts as a novel pathophysiological determinant of modeled rheumatoid arthritis.PLoS genetics · 2005Article
- Tumor suppressor activity of profilin requires a functional actin binding site.Molecular biology of the cell · 2004Article
- Proteomic dissection of dome formation in a mammary cell line: role of tropomyosin-5b and maspin.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Oxamflatin is a novel antitumor compound that inhibits mammalian histone deacetylase.Oncogene · 1999Article
- Severing of F-actin by the amino-terminal half of gelsolin suggests internal cooperativity in gelsolin.Biophysical journal · 1998Article
- Role of phosphatidylinositol 4,5-bisphosphate in Ras/Rac-induced disruption of the cortactin-actomyosin II complex and malignant transformation.Molecular and cellular biology · 1998Article
- Expression of tropomyosin isoforms in benign and malignant human breast lesions.British journal of cancer · 1996Article
- Forced expression of chimeric human fibroblast tropomyosin mutants affects cytokinesis.The Journal of cell biology · 1995Article
- The Drosophila lethal(2)giant larvae tumor suppressor protein forms homo-oligomers and is associated with nonmuscle myosin II heavy chain.The Journal of cell biology · 1994Article
- Distribution of gelsolin in mouse ovary.Cell and tissue research · 1994Article
- Altered shape and cell cycle characteristics of fibroblasts expressing the E2F1 transcription factor.Molecular biology of the cell · 1994Article
- The COOH terminus of the c-Abl tyrosine kinase contains distinct F- and G-actin binding domains with bundling activity.The Journal of cell biology · 1994Article
- Expression of transduced tropomyosin 1 cDNA suppresses neoplastic growth of cells transformed by the ras oncogene.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Purification and properties of transgelin: a transformation and shape change sensitive actin-gelling protein.The Journal of cell biology · 1993Article
- Suppression of tumorigenicity in simian virus 40-transformed 3T3 cells transfected with alpha-actinin cDNA.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- Gas2, a growth arrest-specific protein, is a component of the microfilament network system.The Journal of cell biology · 1992Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A systematic comparison of the protein synthesis patterns of cultured normal and transformed human fibroblasts and epithelial cells, using two-dimensional gel protein analysis combined with computerized imaging and data acquisition, identified a 90-kD protein (SSP 5714) as one of the most striking downregulated markers typical of the transformed state. Using the information stored in the comprehensive human cellular protein database, we found this protein strongly expressed in several fetal tissues and one of them, epidermis, served as a source for preparative two-dimensional gel electrophoresis. Partial amino acid sequences were generated from peptides obtained by in situ digestion of the electroblotted protein. These sequences identified the marker protein as gelsolin, a finding that was confirmed by two-dimensional immunoblotting of human MRC-5 fibroblast proteins using specific antibodies and by coelectrophoresis with purified human gelsolin. These results suggest that an important regulatory protein of the microfilament system may play a role in defining the phenotype of transformed human fibroblast and epithelial cells in culture.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.