ArticleThe Journal of cell biology1990
Protein phosphatase type-1, not type-2A, modulates actin microfilament integrity and myosin light chain phosphorylation in living nonmuscle cells.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Pooled it
- Dendritic spine alterations in schizophrenia.Neuroscience letters · 2015Review
- Cell- and virus-mediated regulation of the barrier-to-autointegration factor's phosphorylation state controls its DNA binding, dimerization, subcellular localization, and antipoxviral activity.Journal of virology · 2014Article
- Mechanisms regulating cilia growth and cilia function in endothelial cells.Cellular and molecular life sciences : CMLS · 2012Article
- Spinophilin is required for normal morphology, Ca(2+) homeostasis and contraction but dispensable for β-adrenergic stimulation of adult cardiomyocytes.Journal of muscle research and cell motility · 2011Article
- Actin cytoskeleton in myofibroblast differentiation: ultrastructure defining form and driving function.Translational research : the journal of laboratory and clinical medicine · 2011Review
- Scapinin, the protein phosphatase 1 binding protein, enhances cell spreading and motility by interacting with the actin cytoskeleton.PloS one · 2009Article
- Reduced dendritic spine density in auditory cortex of subjects with schizophrenia.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2009Article
- Neurabin/protein phosphatase-1 complex regulates dendritic spine morphogenesis and maturation.Molecular biology of the cell · 2005Article
- Protein phosphatases 1 and 2A transiently associate with myosin during the peak rate of secretion from mast cells.Molecular biology of the cell · 2002Article
- Spinophilin regulates the formation and function of dendritic spines.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Vimentin dephosphorylation by protein phosphatase 2A is modulated by the targeting subunit B55.Molecular biology of the cell · 1999Article
- Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Differential localization of myosin and myosin phosphatase subunits in smooth muscle cells and migrating fibroblasts.Molecular biology of the cell · 1997Article
- Rho-stimulated contractility drives the formation of stress fibers and focal adhesions.The Journal of cell biology · 1996Article
- Okadaic acid-induced transcriptional downregulation of type I collagen gene expression is mediated by protein phosphatase 2A.Molecular and cellular biochemistry · 1996Article
- Myosin II filament assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles.The Journal of cell biology · 1995Article
- Expression of a peptide inhibitor of protein phosphatase 1 increases phosphorylation and activity of CREB in NIH 3T3 fibroblasts.Molecular and cellular biology · 1994Article
- ADP-ribosylation of Rho proteins by Clostridium botulinum exoenzyme C3 is influenced by phosphorylation of Rho-associated factors.The Biochemical journal · 1994Article
- Non-sarcomeric mode of myosin II organization in the fibroblast lamellum.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamic reorganization of the actin microfilament networks is dependent on the reversible phosphorylation of myosin light chain. To assess the potential role of protein phosphatases in this process in living nonmuscle cells, we have microinjected the purified type-1 and type-2A phosphatases into the cytoplasm of mammalian fibroblasts. Our studies reveal that elevating type-1 phosphatase levels led to the rapid (within 30 min) and fully reversible disassembly of the actin microfilament network as determined by immunofluorescence analysis. In contrast, microinjection of equivalent amounts of the purified type-2A phosphatase had no effect on actin microfilament organization. Metabolic labeling of cells after injection of purified phosphatases was used to analyze changes in protein phosphorylation. Concomitant with the disassembly of the actin microfilaments induced by type-1 phosphatase, there was an extensive dephosphorylation of myosin light chain. No such change was observed when cells were injected with type-2A phosphatase. In addition, after extraction of fibroblasts with Triton X-100, the type-1 phosphatase could be specifically localized by immunofluorescence to a fibrillar network of microfilaments. Furthermore, neutralizing type-1 phosphatase activity in vivo by microinjection of an affinity-purified antibody, prevented the reorganization of actin microfilaments that we had previously described following injection of cAMP-dependent protein kinase. These data support the notion that type 1 and type-2 phosphatases have distinct substrate specificity in living cells, and that type-1 phosphatase plays a predominant role in the dephosphorylation of myosin light chain and thus in the modulation of actin microfilament organization in vivo in intact nonmuscle cells.
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