Evidence map›Paper›PMID 21640189›Full record

ArticleProtein expression and purification2011

Expression and purification of myristoylated matrix protein of Mason-Pfizer monkey virus for NMR and MS measurements.

Jan Prchal, Petra Junkova, Miroslava Strmiskova, Jan Lipov, Radovan Hynek, Tomas Ruml, Richard Hrabal

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein expression and purification, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Jan PrchalLaboratory of NMR Spectroscopy, Institute of Chemical Technology, Prague, Czech Republic.
Petra Junkova
Miroslava Strmiskova
Jan Lipov
Radovan Hynek
Tomas Ruml
Richard Hrabal
University of Chemistry and Technology, Prague · CZScientific Research Institute of Introscopy · RU

Funding

GENETICS OF PRIMATE D TYPE RETROVIRUSESR01CA027834 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI HUNTER, ERIC · 1985 to 2011
$3.6M
GENETICS OF PRIMATE D TYPE RETROVIRUSESR37CA027834 · NCI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI HUNTER, ERIC · 1991 to 1999
–
NCI NIH HHS CA 27834NCI NIH HHS R01 CA027834NCI NIH HHS R37 CA027834
6 · The paper itself

Abstract

Matrix proteins play multiple roles both in early and late stages of the viral replication cycle. Their N-terminal myristoylation is important for interaction with the host cell membrane during virus budding. We used Escherichia coli, carrying N-myristoyltransferase gene, for the expression of the myristoylated His-tagged matrix protein of Mason-Pfizer monkey virus. An efficient, single-step purification procedure eliminating all contaminating proteins including, importantly, the non-myristoylated matrix protein was designed. The comparison of NMR spectra of matrix protein with its myristoylated form revealed substantial structural changes induced by this fatty acid modification.

Indexed as

AcyltransferasesEscherichia coliGene ExpressionMason-Pfizer monkey virusMyristic AcidNuclear Magnetic Resonance, BiomolecularRecombinant Fusion ProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationViral Matrix ProteinsAcyltransferasesglycylpeptide N-tetradecanoyltransferaseMyristic AcidRecombinant Fusion ProteinsViral Matrix Proteins

Identifiers

PMID21640189
PMCPMC3141108
OpenAlexW1965153747

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.