ArticleBiochimica et biophysica acta2011
Synthesis and characterization of degradable multivalent cationic lipids with disulfide-bond spacers for gene delivery.
Article in Biochimica et biophysica acta, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 76 citations in OpenAlex.
- Navigating the intricate in-vivo journey of lipid nanoparticles tailored for the targeted delivery of RNA therapeutics: a quality-by-design approach.Journal of nanobiotechnology · 2024Review
- Cryo-TEM Reveals the Influence of Multivalent Charge and PEGylation on Shape Transitions in Fluid Lipid Assemblies: From Vesicles to Discs, Rods, and Spheres.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Lipids with negative spontaneous curvature decrease the solubility of the cancer drug paclitaxel in liposomes.The European physical journal. E, Soft matter · 2023Article
- Lipids with negative spontaneous curvature decrease the solubility of the cancer drug paclitaxel in liposomes.bioRxiv : the preprint server for biology · 2023Article
- siRNA Functionalized Lipid Nanoparticles (LNPs) in Management of Diseases.Pharmaceutics · 2022Review
- The Potential of Nanomedicine to Unlock the Limitless Applications of mRNA.Pharmaceutics · 2022Review
- Lipid-mRNA nanoparticles landscape for cancer therapy.Frontiers in bioengineering and biotechnology · 2022Review
- Lipophilic Polyamines as Promising Components of Liposomal Gene Delivery Systems.Pharmaceutics · 2021Review
- Lipid-Nucleic Acid Complexes: Physicochemical Aspects and Prospects for Cancer Treatment.Molecules (Basel, Switzerland) · 2020Review
- Efficient Delivery of Antisense Oligonucleotides Using Bioreducible Lipid Nanoparticles In Vitro and In Vivo.Molecular therapy. Nucleic acids · 2020Article
- Lipid nanoparticle technology for therapeutic gene regulation in the liver.Advanced drug delivery reviews · 2020Review
- Lipidic Aminoglycoside Derivatives: A New Class of Immunomodulators Inducing a Potent Innate Immune Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- A step-wise synthetic approach is necessary to access γ-conjugates of folate: folate-conjugated prodigiosenes.RSC advances · 2019Article
- Lipid-based Liquid Crystalline Films and Solutions for the Delivery of Cargo to Cells.Liquid crystals reviews · 2019Article
- Transfection by cationic gemini lipids and surfactants.MedChemComm · 2018Review
- Engineering liposomal nanoparticles for targeted gene therapy.Gene therapy · 2017Review
- Reductive nanocomplex encapsulation of cRGD-siRNA conjugates for enhanced targeting to cancer cells.International journal of nanomedicine · 2017Article
- Rab11 and Lysotracker Markers Reveal Correlation between Endosomal Pathways and Transfection Efficiency of Surface-Functionalized Cationic Liposome-DNA Nanoparticles.The journal of physical chemistry. B · 2016Article
- Aptamer-functionalized peptide H3CR5C as a novel nanovehicle for codelivery of fasudil and miRNA-195 targeting hepatocellular carcinoma.International journal of nanomedicine · 2016Article
- Quantitative Intracellular Localization of Cationic Lipid-Nucleic Acid Nanoparticles with Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Gene therapy provides powerful new approaches to curing a large variety of diseases, which are being explored in ongoing worldwide clinical trials. To overcome the limitations of viral gene delivery systems, synthetic nonviral vectors such as cationic liposomes (CLs) are desirable. However, improvements of their efficiency at reduced toxicity and a better understanding of their mechanism of action are required. We present the efficient synthesis of a series of degradable multivalent cationic lipids (CMVLn, n=2 to 5) containing a disulfide bond spacer between headgroup and lipophilic tails. This spacer is designed to be cleaved in the reducing milieu of the cytoplasm and thus decrease lipid toxicity. Small angle X-ray scattering demonstrates that the initially formed lamellar phase of CMVLn-DNA complexes completely disappears when reducing agents such as DTT or the biologically relevant reducing peptide glutathione are added to mimic the intracellular milieu. The CMVLs (n=3 to 5) exhibit reduced cytotoxicity and transfect mammalian cells with efficiencies comparable to those of highly efficient non-degradable analogs and benchmark commercial reagents such as Lipofectamine 2000. Thus, our results demonstrate that degradable disulfide spacers may be used to reduce the cytotoxicity of synthetic nonviral gene delivery carriers without compromising their transfection efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.