ArticleAnalytical biochemistry2011
An automated point-of-care system for immunodetection of staphylococcal enterotoxin B.
Article in Analytical biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Microfluidics on lensless, semiconductor optical image sensors: challenges and opportunities for democratization of biosensing at the micro-and nano-scale.Nanophotonics (Berlin, Germany) · 2023Review
- The Role ofInternational journal of molecular sciences · 2022Review
- Sample-to-Answer Robotic ELISA.Analytical chemistry · 2021Article
- Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review.Food science & nutrition · 2020Review
- Rapid detection of staphylococcal enterotoxin B in milk samples based on fluorescence hybridization chain reaction amplification.RSC advances · 2018Article
- Review
- Improving the Sensitivity and Functionality of Mobile Webcam-Based Fluorescence Detectors for Point-of-Care Diagnostics in Global Health.Diagnostics (Basel, Switzerland) · 2016Review
- A Colorimetric Enzyme-Linked Immunosorbent Assay (ELISA) Detection Platform for a Point-of-Care Dengue Detection System on a Lab-on-Compact-Disc.Sensors (Basel, Switzerland) · 2015Article
- Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.The Analyst · 2014Article
- Recent developments in optical detection technologies in lab-on-a-chip devices for biosensing applications.Sensors (Basel, Switzerland) · 2014Article
- Electrical percolation based biosensors.Methods (San Diego, Calif.) · 2013Article
- Optical imaging techniques for point-of-care diagnostics.Lab on a chip · 2013Review
- Optimizing two-color semiconductor nanocrystal immunoassays in single well microtiter plate formats.Sensors (Basel, Switzerland) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
An automated point-of-care (POC) immunodetection system for immunological detection of staphylococcal enterotoxin B (SEB) was designed, fabricated, and tested. The system combines several elements: (i) enzyme-linked immunosorbent assay-lab-on-a-chip (ELISA-LOC) with fluidics, (ii) a charge-coupled device (CCD) camera detector, (iii) pumps and valves for fluid delivery to the ELISA-LOC, (iv) a computer interface board, and (v) a computer for controlling the fluidics, logging, and data analysis of the CCD data. The ELISA-LOC integrates a simple microfluidic system into a miniature 96-well sample plate, allowing the user to carry out immunological assays without a laboratory. The analyte is measured in a sandwich ELISA assay format combined with a sensitive electrochemiluminescence (ECL) detection method. Using the POC system, SEB, a major foodborne toxin, was detected at concentrations as low as 0.1 ng/ml. This is similar to the reported sensitivity of conventional ELISA. The open platform with simple modular fluid delivery automation design described here is interchangeable between detection systems, and because of its versatility it can also be used to automate many other LOC systems, simplifying LOC development. This new POC system is useful for carrying out various immunological and other complex medical assays without a laboratory and can easily be adapted for high-throughput biological screening in remote and resource-poor areas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.