ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2011
High doses of ketamine-xylazine anesthesia reduce cardiac ischemia-reperfusion injury in guinea pigs.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Impact of bicarbonate on ionized calcium and cardiac function in albumin-free Langendorff perfusion in hearts of male rats.Physiological reports · 2026Article
- Molecular basis of the different effects of procainamide and N-acetylprocainamide on the maximum upstroke velocity and half-decay time of the cardiac action potential in guinea pig papillary muscle.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023Article
- Reevaluating methods reporting practices to improve reproducibility: an analysis of methodological rigor for the Langendorff whole heart technique.American journal of physiology. Heart and circulatory physiology · 2022Review
- The role of nitric oxide on the antiarrhythmic effects of ketamine/xylazine in a rat model of acute cardiac ischemia-reperfusion.Current research in physiology · 2022Article
- Anesthesia Protocols used to Create Ischemia Reperfusion Myocardial Infarcts in Swine.Journal of the American Association for Laboratory Animal Science : JAALAS · 2020Review
- Distinctive effect of anesthetics on the effect of limb remote ischemic postconditioning following ischemic stroke.PloS one · 2020Article
- Two-photon microscopy allows imaging and characterization of cochlear microvasculature in vivo.BioMed research international · 2015Article
- Non-terminal blood sampling techniques in guinea pigs.Journal of visualized experiments : JoVE · 2014Article
- Pulmonary instillation of multi-walled carbon nanotubes promotes coronary vasoconstriction and exacerbates injury in isolated hearts.Nanotoxicology · 2014Article
- The effect of intravenous anesthetics on ischemia-reperfusion injury.BioMed research international · 2014Review
- Reduction of early reperfusion injury with the mitochondria-targeting peptide bendavia.Journal of cardiovascular pharmacology and therapeutics · 2014Article
- Stage of the estrous cycle does not influence myocardial ischemia-reperfusion injury in rats (Rattus norvegicus).Comparative medicine · 2013Article
- Combination of ketamine and xylazine exacerbates cardiac dysfunction in severely scalded rats during the shock stage.Experimental and therapeutic medicine · 2013Article
- Comparison of ketamine-xylazine and ketamine-dexmedetomidine anesthesia and intraperitoneal tolerance in rats.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Cardiac output, at rest and during exercise, before and during myocardial ischemia, reperfusion, and infarction in conscious mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Myocardial ischemia, reperfusion, and infarction in chronically instrumented, intact, conscious, and unrestrained mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- Ischemia reperfusion myocardium injuries in type 2 diabetic rats: Effects of ketamine and insulin on LC3-II and mTOR expression.International journal of immunopathology and pharmacologyArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Choosing an appropriate anesthetic protocol that will have minimal effect on experimental design can be difficult. Guinea pigs have highly variable responses to a variety of injectable anesthetics, including ketamine-xylazine (KX). Because of this variability, supplemental doses often are required to obtain an adequate plane of anesthesia. Our group studies the isolated guinea pig heart, and we must anesthetize guinea pigs prior to harvesting this organ. In this study, we sought to determine whether a higher dose of KX protected isolated guinea pig hearts against myocardial ischemia-reperfusion injury. Male Hartley guinea pigs (Crl:HA; 275 to 300 g; n = 14) were anesthetized with either of 2 doses of KX (K: 85 mg/kg, X: 15 mg/kg; or K: 200 mg/kg, X: 60 mg/kg). After thoracotomy, hearts underwent 20 min of ischemia followed by 2 h of reperfusion. The high dose of KX significantly reduced myocardial infarct size as compared with the low dose (36% ± 3% and 51% ± 6%, respectively). Furthermore, the high dose of KX improved hemodynamic function over that associated with the low dose as measured by increases in both left ventricular developed pressure (49 ± 4 and 30 ± 8 mm Hg, respectively) and maximal rate of left ventricular relaxation (-876 ± 70 and -576 ± 120 mm Hg/s, respectively). However, the high dose of KX did not alter the maximal rate of left ventricular contraction or coronary flow. These results suggest that supplementation of KX to ensure an adequate anesthetic plane may introduce unwanted variability in ischemia-reperfusion studies.
Indexed as
Identifiers
21640030PMC3103285W4817160What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.