ArticleMolecules and cells2011
Identification and functional characterization of ion channels in CD34(+) hematopoietic stem cells from human peripheral blood.
Article in Molecules and cells, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- The role and mechanism of vascular wall cell ion channels in vascular fibrosis remodeling.Channels (Austin, Tex.) · 2024Review
- Pathophysiological role of calcium channels and transporters in the multiple myeloma.Cell communication and signaling : CCS · 2021Review
- Distribution and Assembly of TRP Ion Channels.Advances in experimental medicine and biology · 2021Article
- Conditioned Medium from Canine Amniotic Membrane-Derived Mesenchymal Stem Cells Improved Dog Sperm Post-Thaw Quality-Related Parameters.Animals : an open access journal from MDPI · 2020Article
- Altered expression and functional role of ion channels in leukemia: bench to bedside.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2020Review
- Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease.Open life sciences · 2020Article
- TRPV2: A Cancer Biomarker and Potential Therapeutic Target.Disease markers · 2020Review
- Immune aspects of the bi-directional neuroimmune facilitator TRPV1.Molecular biology reports · 2019Review
- Increased expression of transient receptor potential canonical 6 (TRPC6) in differentiating human megakaryocytes.Cell biology international · 2016Article
- Article
- hERG potassium channel blockage by scorpion toxin BmKKx2 enhances erythroid differentiation of human leukemia cells K562.PloS one · 2013Article
- The role of transient receptor potential vanilloid type-2 ion channels in innate and adaptive immune responses.Frontiers in immunology · 2013Article
- The KCNE Tango - How KCNE1 Interacts with Kv7.1.Frontiers in pharmacology · 2012Article
- Ion channels in hematopoietic and mesenchymal stem cells.Stem cells international · 2012Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hematopoietic stem cells (HSCs) are used therapeutically for hematological diseases and may also serve as a source for nonhematopoietic tissue engineering in the future. In other cell types, ion channels have been investigated as potential targets for the regulation of proliferation and differentiation. However, the ion channels of HSCs remain elusive. Here, we functionally characterized the ion channels of CD34(+) cells from human peripheral blood. Using fluorescence-activated cell sorting, we confirmed that the CD34(+) cells also express CD45 and CD133. In the CD34(+)/CD45(+)/CD133(high) HSCs, RT-PCR of 58 ion channel mRNAs revealed the coexpression of Kv1.3, Kv7.1, Nav1.7, TASK2, TALK2, TWIK2, TRPC4, TRPC6, TRPM2, TRPM7, and TRPV2. Whole-cell patch clamp recordings identified voltage-gated K(+) currents (putatively Kv1.3), pH-sensitive TASK2-like back-ground K(+) currents, ADP-ribose-activated TRPM2 currents, temperature-sensitive TRPV2-like currents, and diacylglycerol-analogue-activated TRPC6-like currents. Our results lend new insight into the physiological role of ion channels in HSCs, the specific implications of which require further investigation.
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