ArticlePloS one2011
MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed.
- Functional requirement for Dicer helicase arginine methylation in 26 G siRNA biogenesis and oocyte meiotic program.Nature communications · 2026Article
- Proximity labeling strategies in Caenorhabditis elegans: a comprehensive review.Cell communication and signaling : CCS · 2026Review
- A fluorescence lifetime separation approach for FLIM live-cell imaging.Journal of microscopy · 2026Article
- Intraspecific variation in the duration of epigenetic inheritance.bioRxiv : the preprint server for biology · 2025Article
- FHOD-1 and profilin protect sarcomeres against contraction-induced deformation> inMolecular biology of the cell · 2024Article
- Pachytene piRNAs control discrete meiotic events during spermatogenesis and restrict gene expression in space and time.Science advances · 2024Article
- Phagolysosomes break down the membrane of a non-apoptotic corpse independent of macroautophagy.bioRxiv : the preprint server for biology · 2024Article
- Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in thebioRxiv : the preprint server for biology · 2024Article
- FHOD-1/profilin-mediated actin assembly protects sarcomeres against contraction-induced deformation inbioRxiv : the preprint server for biology · 2024Article
- Tissue-specific silencing of integrated transgenes achieved through endogenous RNA interference inRNA biology · 2024Article
- Phagolysosomes break down the membrane of a non-apoptotic corpse independent of macroautophagy.PloS one · 2024Article
- Subcellular Imaging of Neuronal Calcium Handling In Vivo.Journal of visualized experiments : JoVE · 2023Article
- Analysis of the C. elegans Germline Stem Cell Pool.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Widespread transposon co-option in theScience advances · 2022Article
- Germline immortality relies on TRIM32-mediated turnover of a maternal mRNA activator inScience advances · 2022Article
- A combinedCell cycle (Georgetown, Tex.) · 2022Article
- Modular safe-harbor transgene insertion for targeted single-copy and extrachromosomal array integration in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2022Article
- Dual-expression system for blue fluorescent protein optimization.Scientific reports · 2022Article
- Somatic PMK-1/p38 signaling links environmental stress to germ cell apoptosis and heritable euploidy.Nature communications · 2022Article
- Live-cell Imaging and Analysis of Germline Stem Cell Mitosis inBio-protocol · 2022Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Here we describe a toolkit for the production of fluorescently tagged proteins in the C. elegans germline and early embryo using Mos1-mediated single copy insertion (MosSCI) transformation. We have generated promoter and 3'UTR fusions to sequences of different fluorescent proteins yielding constructs for germline expression that are compatible with MosSCI MultiSite Gateway vectors. These vectors allow tagged transgene constructs to be inserted as single copies into known sites in the C. elegans genome using MosSCI. We also show that two C. elegans heat shock promoters (Phsp-16.2 and Phsp-16.41) can be used to induce transgene expression in the germline when inserted via MosSCI transformation. This flexible set of new vectors, available to the research community in a plasmid repository, should facilitate research focused on the C. elegans germline and early embryo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.