ArticlePloS one2011
Heterologous expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] hydrogenases in Synechococcus elongatus.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Smart-driven bioengineering techniques for enhancing microalgal biohydrogen production.Biotechnology for biofuels and bioproducts · 2026Review
- Co-expression of auxiliary genes enhances the activity of a heterologous OBiotechnology for biofuels and bioproducts · 2025Article
- Novel concepts and engineering strategies for heterologous expression of efficient hydrogenases in photosynthetic microorganisms.Frontiers in microbiology · 2023Review
- Photobiohydrogen Production and Strategies for HAdvances in biochemical engineering/biotechnology · 2023Article
- Inactivation of the uptake hydrogenase in the purple non-sulfur photosynthetic bacterium Rubrivivax gelatinosus CBS enables a biological water-gas shift platform for HJournal of industrial microbiology & biotechnology · 2019Article
- Implementation of photobiological H2 production: the O 2 sensitivity of hydrogenases.Photosynthesis research · 2015Article
- Modeling three-dimensional structure of two closely related Ni-Fe hydrogenases.Photosynthesis research · 2015Article
- Cyanobacterial hydrogenases and hydrogen metabolism revisited: recent progress and future prospects.International journal of molecular sciences · 2015Review
- Genomes of Alteromonas australica, a world apart.BMC genomics · 2014Article
- A broad survey reveals substitution tolerance of residues ligating FeS clusters in [NiFe] hydrogenase.BMC biochemistry · 2014Article
- Circadian rhythms: hijacking the cyanobacterial clock.Current biology : CB · 2013Article
- Circadian yin-yang regulation and its manipulation to globally reprogram gene expression.Current biology : CB · 2013Article
- Dual organism design cycle reveals small subunit substitutions that improve [NiFe] hydrogenase hydrogen evolution.Journal of biological engineering · 2013Article
- Wide-dynamic-range promoters engineered for cyanobacteria.Journal of biological engineering · 2013Article
- Genomes of surface isolates of Alteromonas macleodii: the life of a widespread marine opportunistic copiotroph.Scientific reports · 2012Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxygen-tolerant [NiFe] hydrogenases may be used in future photobiological hydrogen production systems once the enzymes can be heterologously expressed in host organisms of interest. To achieve heterologous expression of [NiFe] hydrogenases in cyanobacteria, the two hydrogenase structural genes from Alteromonas macleodii Deep ecotype (AltDE), hynS and hynL, along with the surrounding genes in the gene operon of HynSL were cloned in a vector with an IPTG-inducible promoter and introduced into Synechococcus elongatus PCC7942. The hydrogenase protein was expressed at the correct size upon induction with IPTG. The heterologously-expressed HynSL hydrogenase was active when tested by in vitro H(2) evolution assay, indicating the correct assembly of the catalytic center in the cyanobacterial host. Using a similar expression system, the hydrogenase structural genes from Thiocapsa roseopersicina (hynSL) and the entire set of known accessory genes were transferred to S. elongatus. A protein of the correct size was expressed but had no activity. However, when the 11 accessory genes from AltDE were co-expressed with hynSL, the T. roseopersicina hydrogenase was found to be active by in vitro assay. This is the first report of active, heterologously-expressed [NiFe] hydrogenases in cyanobacteria.
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