ArticlePloS one2011
OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Spo11: from topoisomerase VI to meiotic recombination initiator.Biochemical Society transactions · 2025Review
- dCas9-SPO11-1 locally stimulates meiotic recombination in rice.Frontiers in plant science · 2025Article
- A genome-wide association study using MyanmarBreeding science · 2024Article
- The plant early recombinosome: a high security complex to break DNA during meiosis.Plant reproduction · 2024Review
- Reconstitution of SPO11-dependent double-strand break formation.bioRxiv : the preprint server for biology · 2024Article
- Molecular mechanisms and regulation of recombination frequency and distribution in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- The meiotic topoisomerase VI B subunit (MTOPVIB) is essential for meiotic DNA double-strand break formation in barley (Hordeum vulgare L.).Plant reproduction · 2023Article
- Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants.Plant biotechnology journal · 2023Article
- Meiosis in crops: from genes to genomes.Journal of experimental botany · 2021Review
- Heterologous Complementation of SPO11-1 and -2 Depends on the Splicing Pattern.International journal of molecular sciences · 2021Article
- Engineering meiotic recombination pathways in rice.Plant biotechnology journal · 2019Review
- Advances Towards How Meiotic Recombination Is Initiated: A Comparative View and Perspectives for Plant Meiosis Research.International journal of molecular sciences · 2019Review
- Role of a cotton endoreduplication-related gene, GaTOP6B, in response to drought stress.Planta · 2019Article
- Functional characterization of the meiosis-specific DNA double-strand break inducing factor SPO-11 from C. elegans.Scientific reports · 2017Article
- Bivalent Formation 1, a plant-conserved gene, encodes an OmpH/coiled-coil motif-containing protein required for meiotic recombination in rice.Journal of experimental botany · 2017Article
- Evolution of meiotic recombination genes in maize and teosinte.BMC genomics · 2017Article
- P31comet, a member of the synaptonemal complex, participates in meiotic DSB formation in rice.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice.The Plant cell · 2016Article
- OsSDS is essential for DSB formation in rice meiosis.Frontiers in plant science · 2015Article
- On the role of AtDMC1, AtRAD51 and its paralogs during Arabidopsis meiosis.Frontiers in plant science · 2014Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA topoisomerase VI from Archaea, a heterotetrameric complex composed of two TopVIA and two TopVIB subunits, is involved in altering DNA topology during replication, transcription and chromosome segregation by catalyzing DNA strand transfer through transient double-strand breaks. The sequenced yeast and animal genomes encode only one homologue of the archaeal TopVIA subunit, namely Spo11, and no homologue of the archaeal TopVIB subunit. In yeast, Spo11 is essential for initiating meiotic recombination and this function appears conserved among other eukaryotes. In contrast to yeast and animals, studies in Arabidopsis and rice have identified three Spo11/TopVIA homologues and one TopVIB homologue in plants. Here, we further identified two novel Spo11/TopVIA homologues (named OsSpo11-4 and OsSpo11-5, respectively) that exist just in the monocot model plant Oryza sativa, indicating that at least five Spo11/TopVIA homologues are present in the rice genome. To reveal the biochemical function of the two novel Spo11/TopVIA homologues, we first examined the interactions among OsSpo11-1, OsSpo11-4, OsSpo11-5, and OsTopVIB by yeast two-hybrid assay. The results showed that OsSpo11-4 and OsTopVIB can self-interact strongly and among the 3 examined OsSpo11 proteins, only OsSpo11-4 interacted with OsTopVIB. Pull-down assay confirmed the interaction between OsSpo11-4 and OsTopVIB, which indicates that OsSpo11-4 may interact with OsTopVIB in vivo. Further in vitro enzymatic analysis revealed that among the above 4 proteins, only OsSpo11-4 exhibited double-strand DNA cleavage activity and its enzymatic activity appears dependent on Mg(2+) and independent of OsTopVIB, despite its interaction with OsTopVIB. We further analyzed the biological function of OsSpo11-4 by RNA interference and found that down-regulated expression of OsSpo11-4 led to defects in male meiosis, indicating OsSpo11-4 is required for meiosis.
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