ArticlePLoS genetics2011
Meiotic recombination intermediates are resolved with minimal crossover formation during return-to-growth, an analogue of the mitotic cell cycle.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 90 citations in OpenAlex.
- Distinct and overlapping roles of MutLγ, Mus81-Mms4, and STR in meiotic Holliday junction processing.Nature communications · 2026Article
- The dynamics of loss of heterozygosity events in genomes.EMBO reports · 2025Review
- Insights into the identification and evolutionary conservation of key genes in the transcriptional circuits of meiosis initiation and commitment in budding yeast.FEBS open bio · 2023Article
- Meiosis in budding yeast.Genetics · 2023Article
- Loss of Heterozygosity and Its Importance in Evolution.Journal of molecular evolution · 2023Review
- Evolution of loss of heterozygosity patterns in hybrid genomes of Candida yeast pathogens.BMC biology · 2023Article
- Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltii.PLoS genetics · 2023Article
- Unlocking the functional potential of polyploid yeasts.Nature communications · 2022Article
- The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells.Science advances · 2022Article
- Aborting meiosis allows recombination in sterile diploid yeast hybrids.Nature communications · 2021Article
- Repeated strand invasion and extensive branch migration are hallmarks of meiotic recombination.Molecular cell · 2021Article
- The synaptonemal complex central region modulates crossover pathways and feedback control of meiotic double-strand break formation.Nucleic acids research · 2021Article
- Loss of heterozygosity results in rapid but variable genome homogenization across yeast genetic backgrounds.eLife · 2021Article
- Loss of Heterozygosity and Base Mutation Rates Vary AmongG3 (Bethesda, Md.) · 2020Article
- C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes ofG3 (Bethesda, Md.) · 2020Article
- Timing of appearance of new mutations during yeast meiosis and their association with recombination.Current genetics · 2020Article
- Review
- High-Throughput Single-Cell Sequencing with Linear Amplification.Molecular cell · 2019Article
- Unresolved Recombination Intermediates Cause aGenetics · 2019Article
- The DNA damage checkpoint and the spindle position checkpoint: guardians of meiotic commitment.Current genetics · 2019Review
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Accurate segregation of homologous chromosomes of different parental origin (homologs) during the first division of meiosis (meiosis I) requires inter-homolog crossovers (COs). These are produced at the end of meiosis I prophase, when recombination intermediates that contain Holliday junctions (joint molecules, JMs) are resolved, predominantly as COs. JM resolution during the mitotic cell cycle is less well understood, mainly due to low levels of inter-homolog JMs. To compare JM resolution during meiosis and the mitotic cell cycle, we used a unique feature of Saccharomyces cerevisiae, return to growth (RTG), where cells undergoing meiosis can be returned to the mitotic cell cycle by a nutritional shift. By performing RTG with ndt80 mutants, which arrest in meiosis I prophase with high levels of interhomolog JMs, we could readily monitor JM resolution during the first cell division of RTG genetically and, for the first time, at the molecular level. In contrast to meiosis, where most JMs resolve as COs, most JMs were resolved during the first 1.5-2 hr after RTG without producing COs. Subsequent resolution of the remaining JMs produced COs, and this CO production required the Mus81/Mms4 structure-selective endonuclease. RTG in sgs1-ΔC795 mutants, which lack the helicase and Holliday junction-binding domains of this BLM homolog, led to a substantial delay in JM resolution; and subsequent JM resolution produced both COs and NCOs. Based on these findings, we suggest that most JMs are resolved during the mitotic cell cycle by dissolution, an Sgs1 helicase-dependent process that produces only NCOs. JMs that escape dissolution are mostly resolved by Mus81/Mms4-dependent cleavage that produces both COs and NCOs in a relatively unbiased manner. Thus, in contrast to meiosis, where JM resolution is heavily biased towards COs, JM resolution during RTG minimizes CO formation, thus maintaining genome integrity and minimizing loss of heterozygosity.
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