ArticlePloS one2011
Combining computational prediction of cis-regulatory elements with a new enhancer assay to efficiently label neuronal structures in the medaka fish.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- MEPD: medaka expression pattern database, genes and more.Nucleic acids research · 2016Article
- Review
- Article
- Genomic and phenotypic characterization of a wild medaka population: towards the establishment of an isogenic population genetic resource in fish.G3 (Bethesda, Md.) · 2014Article
- Article
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
- A living fossil in the genome of a living fossil: Harbinger transposons in the coelacanth genome.Molecular biology and evolution · 2012Article
- A novel mammal-specific three partite enhancer element regulates node and notochord-specific Noto expression.PloS one · 2012Article
- De novo genesis of enhancers in vertebrates.PLoS biology · 2011Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The developing vertebrate nervous system contains a remarkable array of neural cells organized into complex, evolutionarily conserved structures. The labeling of living cells in these structures is key for the understanding of brain development and function, yet the generation of stable lines expressing reporter genes in specific spatio-temporal patterns remains a limiting step. In this study we present a fast and reliable pipeline to efficiently generate a set of stable lines expressing a reporter gene in multiple neuronal structures in the developing nervous system in medaka. The pipeline combines both the accurate computational genome-wide prediction of neuronal specific cis-regulatory modules (CRMs) and a newly developed experimental setup to rapidly obtain transgenic lines in a cost-effective and highly reproducible manner. 95% of the CRMs tested in our experimental setup show enhancer activity in various and numerous neuronal structures belonging to all major brain subdivisions. This pipeline represents a significant step towards the dissection of embryonic neuronal development in vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.