ArticlePloS one2011
Detection of pathogenic mycobacteria based on functionalized quantum dots coupled with immunomagnetic separation.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nanoparticles as an Alternative Strategy for the Rapid Detection ofIET nanobiotechnology · 2025Pooled it
- Rapid and sensitive isolation ofMicrobiology spectrum · 2025Article
- Evolution of tuberculosis diagnostics: From molecular strategies to nanodiagnostics.Tuberculosis (Edinburgh, Scotland) · 2023Review
- Ultrafast and Sensitive Screening of Pathogens by Functionalized Janus Microbeads-Enabled Rotational Diffusometry in Combination with Isothermal Amplification.Small science · 2022Article
- Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis.Biosensors · 2018Article
- A Rapid Detection Method of Brucella with Quantum Dots and Magnetic Beads Conjugated with Different Polyclonal Antibodies.Nanoscale research letters · 2017Article
- Detection of Mycobacterium tuberculosis based on H37R(v) binding peptides using surface functionalized magnetic microspheres coupled with quantum dots – a nano detection method for Mycobacterium tuberculosis.International journal of nanomedicine · 2015Article
- Prevention of bacterial foodborne disease using nanobiotechnology.Nanotechnology, science and applications · 2014Review
- Immunomagnetic separation combined with polymerase chain reaction for the detection of Alicyclobacillus acidoterrestris in apple juice.PloS one · 2013Article
- Immunomagnetic nanoparticle-based assays for detection of biomarkers.International journal of nanomedicine · 2013Review
- Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection.World journal of gastroenterology · 2012Article
- An IMS/ATP Assay for the Detection of Mycobacterium tuberculosis in Urine.Tuberculosis research and treatment · 2012Article
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Authors and funding
6 authors.
Funding
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Abstract
Mycobacteria have always proven difficult to identify due to their low growth rate and fastidious nature. Therefore molecular biology and more recently nanotechnology, have been exploited from early on for the detection of these pathogens. Here we present the first stage of development of an assay incorporating cadmium selenide quantum dots (QDs) for the detection of mycobacterial surface antigens. The principle of the assay is the separation of bacterial cells using magnetic beads coupled with genus-specific polyclonal antibodies and monoclonal antibodies for heparin-binding hemagglutinin. These complexes are then tagged with anti-mouse biotinylated antibody and finally streptavidin-conjugated QDs which leads to the detection of a fluorescent signal. For the evaluation of performance, the method under study was applied on Mycobacterium bovis BCG and Mycobacterium tuberculosis (positive controls), as well as E. coli and Salmonella spp. that constituted the negative controls. The direct observation of the latter category of samples did not reveal fluorescence as opposed to the mycobacteria mentioned above. The minimum detection limit of the assay was defined to 10(4) bacteria/ml, which could be further decreased by a 1 log when fluorescence was measured with a spectrofluorometer. The method described here can be easily adjusted for any other protein target of either the pathogen or the host, and once fully developed it will be directly applicable on clinical samples.
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