ArticleGenetics and molecular biology2010
Molecular cloning and expression of key gene encoding hypothetical DNA polymerase from B. mori parvo-like virus.
Article in Genetics and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Parvovirus dark matter in the cloaca of wild birds.GigaScience · 2022Article
- Characterization of the RNA Transcription Profile ofViruses · 2019Article
- Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus.Virology journal · 2016Article
- Determination of the proteins encoded by BmBDV VD1-ORF4 and their interacting proteins in BmBDV-infected midguts.Current microbiology · 2015Article
- Identification of Bombyx mori bidensovirus VD1-ORF4 reveals a novel protein associated with viral structural component.Current microbiology · 2013Article
- Characterization of the promoter elements of Bombyx mori bidensovirus nonstructural gene 1.Current microbiology · 2012Article
- The non-structural protein NS-2 of Bombyx mori parvo-like virus is localized to the nuclear membrane.Current microbiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BmPLV-Z is the abbreviation for Bombyx mori parvo-like virus (China isolate). This is a novel virus with two single-stranded linear DNA molecules, viz., VD1 (6543 bp) and VD2 (6022 bp), which are encapsidated respectively into separate virions. Analysis of the deduced amino acid sequence of VD1-ORF4 indicated the existence of a putative DNA-polymerase with exonuclease activity, possibly involved in the replication of BmPLV-Z. In the present study, a recombinant baculovirus was constructed to express the full length of the protein encoded by the VD1-ORF4 gene (3318 bp). In addition, a 2163-bp fragment amplified from the very same gene was cloned into prokaryotic expression vector pET-30a and expressed in E.coli Rosetta 2 (DE3) pLysS. The expressed fusion protein was employed to immunize New Zealand white rabbits for the production of an antiserum, afterwards used for examining the expression of the protein encoded by VD1-ORF4 gene in Sf-9 cells infected with recombinant baculovirus. Western blot analysis of extracts from thus cells infected revealed a specific band of about 120 kDa, thereby indicating that the full length protein encoded by the VD1-ORF4 gene had been successfully and stably expressed in Sf-9 cells.
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Registered trials
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