ArticlePloS one2011
Allele-specific, age-dependent and BMI-associated DNA methylation of human MCHR1.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Epigenetics and human obesity.International journal of obesity (2005) · 2015Pooled it
- Cord Blood DNA Methylation and Large-for-Gestational-Age Birth: A Pilot Epigenome-Wide Study in the GROW Cohort.Epigenomes · 2026Article
- The Epigenetics of Psychosis: A Structured Review with Representative Loci.Biomedicines · 2022Review
- DNA methylation markers in obesity, metabolic syndrome, and weight loss.Epigenetics · 2019Review
- Oncoprotein CIP2A promotes the disassembly of primary cilia and inhibits glycolytic metabolism.EMBO reports · 2018Article
- A histone-mimicking interdomain linker in a multidomain protein modulates multivalent histone binding.The Journal of biological chemistry · 2017Article
- Integrated transcriptome and methylome analysis in youth at high risk for bipolar disorder: a preliminary analysis.Translational psychiatry · 2017Article
- Tissue-specific methylation profile in obese patients with type 2 diabetes before and after Roux-en-Y gastric bypass.Diabetology & metabolic syndrome · 2017Review
- Epigenomic profiling of preterm infants reveals DNA methylation differences at sites associated with neural function.Translational psychiatry · 2016Article
- Association between DNA Methylation in Whole Blood and Measures of Glucose Metabolism: KORA F4 Study.PloS one · 2016Article
- Impact of Maternal Diet on the Epigenome during In Utero Life and the Developmental Programming of Diseases in Childhood and Adulthood.Nutrients · 2015Review
- Epigenomics, gestational programming and risk of metabolic syndrome.International journal of obesity (2005) · 2015Review
- Genome-wide blood DNA methylation alterations at regulatory elements and heterochromatic regions in monozygotic twins discordant for obesity and liver fat.Clinical epigenetics · 2015Article
- DNA methylation and single nucleotide variants in the brain-derived neurotrophic factor (BDNF) and oxytocin receptor (OXTR) genes are associated with anxiety/depression in older women.Frontiers in genetics · 2015Article
- Epigenomics of Alzheimer's disease.Translational research : the journal of laboratory and clinical medicine · 2015Review
- Hypermethylation in the ZBTB20 gene is associated with major depressive disorder.Genome biology · 2014Article
- Narrowing down the distal border of the copy number variable beta-defensin gene cluster on human 8p23.BMC research notes · 2014Article
- Allele-specific methylation occurs at genetic variants associated with complex disease.PloS one · 2014Article
- (Epi)Genetic analyses of age-related macular degeneration: case-control and discordant twin studies.Human heredity · 2014Article
- High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers.PloS one · 2013Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMelanin-concentrating hormone receptor 1 (MCHR1) plays a significant role in regulation of energy balance, food intake, physical activity and body weight in humans and rodents. Several association studies for human obesity showed contrary results concerning the SNPs rs133072 (G/A) and rs133073 (T/C), which localize to the first exon of MCHR1. The variations constitute two main haplotypes (GT, AC). Both SNPs affect CpG dinucleotides, whereby each haplotype contains a potential methylation site at one of the two SNP positions. In addition, 15 CpGs in close vicinity of these SNPs constitute a weak CpG island. Here, we studied whether DNA methylation in this sequence context may contribute to population- and age-specific effects of MCHR1 alleles in obesity. PRINCIPAL
findingsWe analyzed DNA methylation of a 315 bp region of MCHR1 encompassing rs133072 and rs133073 and the CpG island in blood samples of 49 individuals by bisulfite sequencing. The AC haplotype shows a significantly higher methylation level than the GT haplotype. This allele-specific methylation is age-dependent. In young individuals (20-30 years) the difference in DNA methylation between haplotypes is significant; whereas in individuals older than 60 years it is not detectable. Interestingly, the GT allele shows a decrease in methylation status with increasing BMI, whereas the methylation of the AC allele is not associated with this phenotype. Heterozygous lymphoblastoid cell lines show the same pattern of allele-specific DNA methylation. The cell line, which exhibits the highest difference in methylation levels between both haplotypes, also shows allele-specific transcription of MCHR1, which can be abolished by treatment with the DNA methylase inhibitor 5-aza-2'-deoxycytidine.
conclusionsWe show that DNA methylation at MCHR1 is allele-specific, age-dependent, BMI-associated and affects transcription. Conceivably, this epigenetic regulation contributes to the age- and/or population specific effects reported for MCHR1 in several human obesity studies.
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