ArticleNature protocols2011
Generation of phospholipid vesicle-nanotube networks and transport of molecules therein.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The trial behind it
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Who cites it
18 citing papers in PubMed.
- Microrail-assisted liposome trapping and aligning in microfluidic channels.RSC advances · 2024Article
- SecYEG-mediated translocation in a model synthetic cell.Synthetic biology (Oxford, England) · 2024Article
- Identifying and Manipulating Giant Vesicles: Review of Recent Approaches.Micromachines · 2022Review
- Nonlinear material and ionic transport through membrane nanotubes.Biochimica et biophysica acta. Biomembranes · 2021Article
- Dissipative self-assembly, competition and inhibition in a self-reproducing protocell model.Chemical science · 2020Article
- Biological lipid nanotubes and their potential role in evolution.The European physical journal. Special topics · 2020Review
- Physicochemical considerations for bottom-up synthetic biology.Emerging topics in life sciences · 2019Article
- Combining patch-clamping and fluorescence microscopy for quantitative reconstitution of cellular membrane processes with Giant Suspended Bilayers.Scientific reports · 2019Article
- Mechanics of the Formation, Interaction, and Evolution of Membrane Tubular Structures.Biophysical journal · 2019Article
- Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients.Journal of visualized experiments : JoVE · 2018Article
- Sculpting and fusing biomimetic vesicle networks using optical tweezers.Nature communications · 2018Article
- Soft Nanotubes Derivatized with Short PEG Chains for Thermally Controllable Extraction and Separation of Peptides.ACS omega · 2017Article
- Placing and shaping liposomes with reconfigurable DNA nanocages.Nature chemistry · 2017Article
- An engineered dimeric protein pore that spans adjacent lipid bilayers.Nature communications · 2013Article
- Electric moulding of dispersed lipid nanotubes into a nanofluidic device.Scientific reports · 2013Article
- Nanobiochips.Cellular and molecular life sciences : CMLS · 2012Review
- Nanofluidics: Neither shaken nor stirred.Nature nanotechnology · 2011Article
- Formation of oligovesicular vesicles by micromanipulation.Membranes · 2011Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe micromanipulation and microinjection procedures for the fabrication of soft-matter networks consisting of lipid bilayer nanotubes and surface-immobilized vesicles. These biomimetic membrane systems feature unique structural flexibility and expandability and, unlike solid-state microfluidic and nanofluidic devices prepared by top-down fabrication, they allow network designs with dynamic control over individual containers and interconnecting conduits. The fabrication is founded on self-assembly of phospholipid molecules, followed by micromanipulation operations, such as membrane electroporation and microinjection, to effect shape transformations of the membrane and create a series of interconnected compartments. Size and geometry of the network can be chosen according to its desired function. Membrane composition is controlled mainly during the self-assembly step, whereas the interior contents of individual containers is defined through a sequence of microneedle injections. Networks cannot be fabricated with other currently available methods of giant unilamellar vesicle preparation (large unilamellar vesicle fusion or electroformation). Described in detail are also three transport modes, which are suitable for moving water-soluble or membrane-bound small molecules, polymers, DNA, proteins and nanoparticles within the networks. The fabrication protocol requires ∼90 min, provided all necessary preparations are made in advance. The transport studies require an additional 60-120 min, depending on the transport regime.
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21637199What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.