ArticleNature protocols2011
Initiation, growth and cryopreservation of plant cell suspension cultures.
Article in Nature protocols, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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Who cites it
55 citing papers in PubMed.
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- In vitro bioactive metabolite production and plant regeneration of medicinal plant Ligusticum chuanxiong Hort.BMC plant biology · 2026Article
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- Advanced Solubilization of Brazilian Cerrado Byproduct Extracts Using Green Nanostructured Lipid Carriers and NaDESs for Enhanced Antioxidant Potentials.Antioxidants (Basel, Switzerland) · 2025Article
- From "Maraschino" to Cell Cultures: A Deep Study onMolecules (Basel, Switzerland) · 2025Article
- Impact ofBiotech (Basel (Switzerland)) · 2025Article
- Performance of Malaysian kenafBioengineered · 2023Article
- Plant regeneration through somatic embryogenesis in cell suspensions of Cenchrus ciliaris L.Plant methods · 2023Article
- Phytosulfokine contributes to suspension culture of Cunninghamia lanceolata through its impact on redox homeostasis.BMC plant biology · 2023Article
- Efficacy ofMicroorganisms · 2023Article
- Enzymatic Antioxidant System Activation Assures the Viability ofPlants (Basel, Switzerland) · 2023Article
- Genome-wide identification of NAC transcription factors and regulation of monoterpenoid indole alkaloid biosynthesis inFrontiers in plant science · 2023Article
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- Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures.Bioresources and bioprocessing · 2022Article
- Use of Different Organic Carbon Sources inPlants (Basel, Switzerland) · 2022Article
- Root-associated bacteria modulate the specialised metabolome ofFrontiers in plant science · 2022Article
- Sustainable utilization of Gyrinops walla Gaetner: in vitro production of sesquiterpenes by chemical and biological elicitation.Journal, genetic engineering & biotechnology · 2021Article
- Production of bioactive plant secondary metabolites through in vitro technologies-status and outlook.Applied microbiology and biotechnology · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methods described in this paper are confined to in vitro dedifferentiated plant cell suspension cultures, which are convenient for the large-scale production of fine chemicals in bioreactors and for the study of cellular and molecular processes, as they offer the advantages of a simplified model system for the study of plants when compared with plants themselves or differentiated plant tissue cultures. The commonly used methods of initiation of a callus from a plant and subsequent steps from callus to cell suspension culture are presented in the protocol. This is followed by three different techniques for subculturing (by weighing cells, pipetting and pouring cell suspension) and four methods for growth measurement (fresh- and dry-weight cells, dissimilation curve and cell volume after sedimentation). The advantages and disadvantages of the methods are discussed. Finally, we provide a two-step (controlled rate) freezing technique also known as the slow (equilibrium) freezing method for long-term storage, which has been applied successfully to a wide range of plant cell suspension cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.