ArticleBioengineered bugs
The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics.
Article in Bioengineered bugs. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- Expression and Characterization of Recombinant Triple Laterosporulin in Chlamydomonas reinhardtii.Probiotics and antimicrobial proteins · 2026Article
- The First Introduction of an Exogenous 5' Untranslated Region for Control of Plastid Transgene Expression in Chlamydomonas reinhardtii.Molecular biotechnology · 2025Article
- An animal-type NaFrontiers in plant science · 2025Article
- Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae.Microbial biotechnology · 2024Review
- The synthetic future of algal genomes.Cell genomics · 2024Review
- Microalgae share key features with human erythrocytes and can safely circulate through the vascular system in mice.Applied microbiology and biotechnology · 2023Article
- Cloning and expression of an anti-cancerous cytokine: human IL-29 gene in Chlamydomonas reinhardtii.AMB Express · 2023Article
- Review
- Unassembled cell wall proteins form aggregates in the extracellular space of Chlamydomonas reinhardtii strain UVM4.Applied microbiology and biotechnology · 2022Article
- Advances in delivery methods ofBioengineered · 2022Review
- OverexpressingMarine drugs · 2022Article
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Genetic Engineering of Microalgae for Secondary Metabolite Production: Recent Developments, Challenges, and Future Prospects.Frontiers in bioengineering and biotechnology · 2022Review
- Recombinant Spider Silk: Promises and Bottlenecks.Frontiers in bioengineering and biotechnology · 2022Review
- Expression of Anti-Lipopolysaccharide Factor Isoform 3 inMarine drugs · 2021Article
- Expression of Recombinant PfCelTOS Antigen in the Chloroplast of Chlamydomonas reinhardtii and its Potential Use in Detection of Malaria.Molecular biotechnology · 2019Article
- Biosynthesis and Secretion of Human Tissue Kallikrein in TransgenicMarine drugs · 2018Article
- Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression.The Plant journal : for cell and molecular biology · 2017Article
- Building a multipurpose insertional mutant library for forward and reverse genetics inPlant methods · 2017Article
Corrections and comments
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2 authors.
Funding
Abstract
Microalgae are a diverse group of eukaryotic photosynthetic microorganisms. While microalgae play a crucial role in global carbon fixation and oxygen evolution, these organisms have recently gained much attention for their potential role in biotechnological and industrial applications, such as the production of biofuels. We investigated the potential of the microalga Chlamydomonas reinhardtii to be a platform for the production of human therapeutic proteins. C. reinhardtii is a unicellular freshwater green alga that has served as a popular model alga for physiological, molecular, biochemical and genetic studies. As such, the molecular toolkit for this microorganism is highly developed, including well-established methods for genetic transformation and recombinant gene expression. We transformed the chloroplast genome of C. reinhardtii with seven unrelated genes encoding for current or potential human therapeutic proteins and found that four of these genes supported protein accumulation to levels that are sufficient for commercial production. Furthermore, the algal-produced proteins were bioactive. Thus, the microalga C. reinhardtii has the potential to be a robust platform for human therapeutic protein production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.