ArticleBMC genomics2011
Diverse and tissue-enriched small RNAs in the plant pathogenic fungus, Magnaporthe oryzae.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 127 citations in OpenAlex.
- Methylation of mycovirus DNA is mediated by the RNAi machinery in vegetative hyphae of Fusarium graminearum.Nucleic acids research · 2025Article
- Fungal small RNA: unveiling the breakthroughs and promising applications.Frontiers in fungal biology · 2025Review
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- The Molecular Mechanisms Employed by the ParasiteInternational journal of molecular sciences · 2023Article
- Discovery of long non-coding RNAs in Aspergillus flavus response to water activity, COScientific reports · 2023Article
- Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction.Molecular plant pathology · 2023Article
- The Biological Roles ofBiomolecules · 2023Review
- Understanding the Dynamics of Blast Resistance in Rice-Journal of fungi (Basel, Switzerland) · 2022Review
- Exploring the Effectiveness and Durability of Trans-Kingdom Silencing of Fungal Genes in the Vascular PathogenInternational journal of molecular sciences · 2022Article
- Comparative profiling of canonical and non-canonical small RNAs in the rice blast fungus,Frontiers in microbiology · 2022Article
- The production and secretion of tRNA-derived RNA fragments in the corn smut fungusFrontiers in fungal biology · 2022Article
- Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Role of Dicer-Dependent RNA Interference in Regulating Mycoparasitic Interactions.Microbiology spectrum · 2021Article
- The stem rust fungus Puccinia graminis f. sp. tritici induces centromeric small RNAs during late infection that are associated with genome-wide DNA methylation.BMC biology · 2021Article
- Article
- Identification of virulence associated milRNAs and their bidirectional targets in Rhizoctonia solani and maize during infection.BMC plant biology · 2021Article
- Comparative Analysis of Transcriptome and sRNAs Expression Patterns in theInternational journal of molecular sciences · 2021Article
- Article
- A Novel DCL2-Dependent Micro-Like RNAFrontiers in microbiology · 2021Article
- The Evolutionary Significance of RNAi in the Fungal Kingdom.International journal of molecular sciences · 2020Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEmerging knowledge of the impact of small RNAs as important cellular regulators has prompted an explosion of small transcriptome sequencing projects. Although significant progress has been made towards small RNA discovery and biogenesis in higher eukaryotes and other model organisms, knowledge in simple eukaryotes such as filamentous fungi remains limited.
resultsHere, we used 454 pyrosequencing to present a detailed analysis of the small RNA transcriptome (~ 15 - 40 nucleotides in length) from mycelia and appressoria tissues of the rice blast fungal pathogen, Magnaporthe oryzae. Small RNAs mapped to numerous nuclear and mitochondrial genomic features including repetitive elements, tRNA loci, rRNAs, protein coding genes, snRNAs and intergenic regions. For most elements, small RNAs mapped primarily to the sense strand with the exception of repetitive elements to which small RNAs mapped in the sense and antisense orientation in near equal proportions. Inspection of the small RNAs revealed a preference for U and suppression of C at position 1, particularly for antisense mapping small RNAs. In the mycelia library, small RNAs of the size 18 - 23 nt were enriched for intergenic regions and repetitive elements. Small RNAs mapping to LTR retrotransposons were classified as LTR retrotransposon-siRNAs (LTR-siRNAs). Conversely, the appressoria library had a greater proportion of 28 - 35 nt small RNAs mapping to tRNA loci, and were classified as tRNA-derived RNA fragments (tRFs). LTR-siRNAs and tRFs were independently validated by 3' RACE PCR and northern blots, respectively.
conclusionsOur findings suggest M. oryzae small RNAs differentially accumulate in vegetative and specialized-infection tissues and may play an active role in genome integrity and regulating growth and development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.