ArticleThe Journal of general physiology1990
Divalent cation activation and inhibition of single calcium release channels from sheep cardiac sarcoplasmic reticulum.
Article in The Journal of general physiology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 110 citations in OpenAlex.
- The biophysical properties of TRIC-A and TRIC-B and their interactions with RyR2.The Journal of general physiology · 2023Article
- EuBiophysical journal · 2023Article
- Statin activation of skeletal ryanodine receptors (RyR1) is a class effect but separable from HMG-CoA reductase inhibition.British journal of pharmacology · 2022Article
- The V2475F CPVT1 mutation yields distinct RyR2 channel populations that differ in their responses to cytosolic CaThe Journal of physiology · 2021Article
- Molecular basis for allosteric regulation of the type 2 ryanodine receptor channel gating by key modulators.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Simvastatin activates single skeletal RyR1 channels but exerts more complex regulation of the cardiac RyR2 isoform.British journal of pharmacology · 2018Article
- Dysregulated ZnThe Journal of biological chemistry · 2017Article
- Intracellular Zinc Modulates Cardiac Ryanodine Receptor-mediated Calcium Release.The Journal of biological chemistry · 2015Article
- Ryanodine receptor gating controls generation of diastolic calcium waves in cardiac myocytes.The Journal of general physiology · 2015Article
- Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp.Cell calcium · 2014Article
- Type 2 ryanodine receptors are highly sensitive to alcohol.FEBS letters · 2014Article
- Patch-clamp electrophysiology of intracellular Ca2+ channels.Cold Spring Harbor protocols · 2013Article
- Luminal Ca2+ controls activation of the cardiac ryanodine receptor by ATP.The Journal of general physiology · 2012Article
- Ca²+-dependent phosphorylation of RyR2 can uncouple channel gating from direct cytosolic Ca²+ regulation.The Journal of membrane biology · 2011Article
- Differential sensitivity of Ca²+ wave and Ca²+ spark events to ruthenium red in isolated permeabilised rabbit cardiomyocytes.The Journal of physiology · 2010Article
- Modulation of the local SR Ca2+ release by intracellular Mg2+ in cardiac myocytes.The Journal of general physiology · 2008Article
- Single-channel characterization of the rabbit recombinant RyR2 reveals a novel inactivation property of physiological concentrations of ATP.The Journal of membrane biology · 2008Article
- Calcium activation of ryanodine receptor channels--reconciling RyR gating models with tetrameric channel structure.The Journal of general physiology · 2005Article
- Termination of cardiac Ca(2+) sparks: an investigative mathematical model of calcium-induced calcium release.Biophysical journal · 2002Article
- Thermodynamically irreversible gating of ryanodine receptors in situ revealed by stereotyped duration of release in Ca(2+) sparks.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Single Ca2+ release channels from vesicles of sheep cardiac junctional sarcoplasmic reticulum have been incorporated into uncharged planar lipid bilayers. Single-channel currents were recorded from Ca2(+)-activated channels that had a Ca2+ conductance of approximately 90 pS. Channel open probability increased sublinearly as the concentration of free Ca2+ was raised at the myoplasmic face, and without additional agonists the channels could not be fully activated even by 100 microM free Ca2+. Lifetime analysis revealed a minimum of two open and three closed states, and indicates that Ca2+ activated the channels by interacting with at least one of the closed states to increase the rate of channel opening. Correlations between adjacent lifetimes suggested there were at least two pathways between the open- and closed-state aggregates. An analysis of bursting behavior also revealed correlations between successive burst lengths and the number of openings per burst. The latter had two geometric components, providing additional evidence for at least two open states. One component appeared to comprise unit bursts, and the lifetime of most of these fell within the dominant shorter open-time distribution associated with over 90% of all openings. A cyclic gating scheme is proposed, with channel activation regulated by the binding of Ca2+ to a closed conformation of the channel protein. Mg2+ may inhibit activation by competing for this binding site, but lifetime and fluctuation analysis suggested that once activated the channels continue to gate normally.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.