ArticleTropical animal health and production2012
PCR test for detecting Taenia solium cysticercosis in pig carcasses.
Article in Tropical animal health and production, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- The Frequency of Porcine Cysticercosis and Factors Associated withPathogens (Basel, Switzerland) · 2024Article
- Nexus between prevalence of Taenia solium (Cysticercus cellulosae) in pigs and retrospective study of humans cysticercosis cases in Benin hospitals.Veterinary research communications · 2024Article
- From laboratory to clinical practice: an update of the immunological and molecular tools for neurocysticercosis diagnosis.Frontiers in parasitology · 2024Review
- Serological and molecular detection of neurocysticercosis among epileptic patients in Nagpur, Maharashtra state (India).Helminthologia · 2023Article
- Prevalence of taeniosis in pig farming community in Andhra Pradesh, India.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Sub-lineages of Taenia solium Asian Genotype Recorded in North India.Acta parasitologica · 2022Article
- DNA-Detection Based Diagnostics forJournal of parasitology research · 2020Review
- Prevalence and distribution of Taenia solium cysticercosis in naturally infected pigs in Punjab, India.PLoS neglected tropical diseases · 2018Article
- Identification and Control of Sources ofJournal of veterinary research · 2018Article
- Optimization of In-House Indirect-ELISA & EITB Assays EmployingIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.
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