ArticleMolecular biology reports2012
Selection of housekeeping genes for normalization of RT-PCR in hypoxic neural stem cells of rat in vitro.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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24 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
- Comparative Gill Transcriptomics Reveals Unresolved Inflammation Under Chronic Hypoxia and Molecular Plasticity During Cyclic Hypoxia inAnimals : an open access journal from MDPI · 2026Article
- Evaluation of reference genes for qPCR in human liver and kidney tissue from individuals with obesity.Scientific reports · 2025Article
- mRNA Abundance of Neurogenic Factors Correlates with Hearing Capacity in Auditory Brainstem Nuclei of the Rat.Life (Basel, Switzerland) · 2023Article
- Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells.BMC molecular and cell biology · 2023Article
- Alterations of housekeeping proteins in human aged and diseased hearts.Pflugers Archiv : European journal of physiology · 2021Article
- Expression of the Tau Protein and Amyloid Protein Precursor Processing Genes in the CA3 Area of the Hippocampus in the Ischemic Model of Alzheimer's Disease in the Rat.Molecular neurobiology · 2020Article
- Statistical differences resulting from selection of stable reference genes after hypoxia and hypothermia in the neonatal rat brain.PloS one · 2020Article
- β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused byFrontiers in microbiology · 2019Article
- PACAP-PAC1 Receptor Activation Is Necessary for the Sympathetic Response to Acute Intermittent Hypoxia.Frontiers in neuroscience · 2019Article
- Nicotine protects rat hypoglossal motoneurons from excitotoxic death via downregulation of connexin 36.Cell death & disease · 2017Article
- Easy-to-use strategy for reference gene selection in quantitative real-time PCR experiments.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Dysregulation of Autophagy, Mitophagy, and Apoptotic Genes in the Medial Temporal Lobe Cortex in an Ischemic Model of Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2016Article
- Reference Genes for Addressing Gene Expression of Bladder Cancer Cell Models under Hypoxia: A Step Towards Transcriptomic Studies.PloS one · 2016Article
- Dysregulation of Amyloid-β Protein Precursor, β-Secretase, Presenilin 1 and 2 Genes in the Rat Selectively Vulnerable CA1 Subfield of Hippocampus Following Transient Global Brain Ischemia.Journal of Alzheimer's disease : JAD · 2015Article
- Aromatic-turmerone induces neural stem cell proliferation in vitro and in vivo.Stem cell research & therapy · 2014Article
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- Validation of housekeeping genes in the brains of rats submitted to chronic intermittent hypoxia, a sleep apnea model.PloS one · 2014Article
- Methamphetamine alters reference gene expression in nigra and striatum of adult rat brain.Neurotoxicology · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression analysis under various conditions using real-time reverse transcription polymerase chain reaction (RT-PCR) needs reliable control genes. Housekeeping genes are commonly used as the control. However, no validated housekeeping gene is available for study of hypoxic neural stem cell culture. To choose appropriate internal control genes, the expression of eight commonly used housekeeping genes was examined in rat neural stem cell model to find one or more stably expressed genes under hypoxic/ischemic conditions. Two genes, HPRT and RPL13A were identified as the most confidential housekeeping genes in this research by geNorm and NormFinder softwares. As a groundwork, the most stable housekeeping genes for neural stem cells under hypoxic/ischemic conditions are initially investigated and validated in this experiment, which might provide a better understanding for the gene expression study in ischemic and necrotic neural stem cell cultures or in ischemic diseases of the central nervous system (CNS).
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