ArticleMolecular biology reports2012
Selection of reference genes for quantitative real-time RT-PCR analysis in citrus.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Effects of Hot Water and Plant Growth Regulator Treatments on Bud Germination and Pathogen Elimination in Citrus Scions.Plants (Basel, Switzerland) · 2026Article
- Selection of stable reference genes in prunus persica fruit infected with monilinia laxa for normalisation of RT-qPCR gene expression data.Scientific reports · 2025Article
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- Metabolomic Profiling Reveals the Quality Variations inFoods (Basel, Switzerland) · 2024Article
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Transcriptomic alterations in the sweet orange vasculature correlate with growth repression induced by a variant of citrus tristeza virus.Frontiers in microbiology · 2023Article
- Role of long non-coding RNA in regulatory network response toFrontiers in plant science · 2023Article
- A chromosome-level phased genome enabling allele-level studies in sweet orange: a case study on citrus Huanglongbing tolerance.Horticulture research · 2023Article
- Genome-Wide Screening and Stability Verification of the Robust Internal Control Genes for RT-qPCR in Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2022Article
- Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata.Scientific reports · 2021Article
- Validation of Reference Genes for Quantitative Real-Time PCR Normalization inFrontiers in genetics · 2021Article
- Citron C-05 inhibits both the penetration and colonization ofHorticulture research · 2020Article
- Selection and validation of appropriate reference genes for gene expression studies inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Identification of Appropriate Reference Genes for Normalizing miRNA Expression in Citrus Infected byGenes · 2019Article
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- Novel Plastid-Nuclear Genome Combinations Enhance Resistance to Citrus Canker in Cybrid Grapefruit.Frontiers in plant science · 2018Article
- Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa.PloS one · 2018Article
- Transcriptional analysis of sweet orange trees co-infected with 'Candidatus Liberibacter asiaticus' and mild or severe strains of Citrus tristeza virus.BMC genomics · 2017Article
- Rootstock influence on iron uptake responses inPeerJ · 2017Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time reverse transcription polymerase chain reaction (qPCR) has become the preferred method for studying low-abundant mRNA expression. Appropriate application of qPCR in such studies requires the use of reference gene(s) as an internal control in order to normalize the mRNA levels between different samples for an exact comparison of gene expression levels. Expression of the reference gene should be independent from development stage, cell/tissue types, treatments and environmental conditions. Recognizing the importance of reference gene(s) in normalization of qPCR data, various reference genes have been evaluated for stable expression under specific conditions in various organisms. In plants, only a few of them have been investigated, and very few reports about such reference genes in citrus. In the present study, seven candidate reference genes (18SrRNA, ACTB, rpII, UBQI, UBQ10, GAPDH and TUB) were tested, and three of them (18SrRNA, ACTB and rpII) proved to be the most stable ones among six leaf samples of different citrus genotypes. The three candidate reference genes were further analyzed for their stability of expression in five different tissues, and the results indicated that they were not completely stable. It is commonly accepted that gene expression studies should be normalized using more than one reference gene. Based on our results, we propose the use of the mean result rendered by18SrRNA, ACTB and rpII as reference genes to normalize mRNA levels in qPCR analysis of diverse cultivars and tissues of citrus. These results may provide a guideline for future works on gene expression in citrus by using qPCR.
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