ArticleThe Journal of biological chemistry2011
Plant pathogenic bacteria utilize biofilm growth-associated repressor (BigR), a novel winged-helix redox switch, to control hydrogen sulfide detoxification under hypoxia.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 83 citations in OpenAlex.
- Genomic and phenotypic characterization of indigenous violacein-producing Chromobacterium amazonense SAM215 from lake sediment in Bangladesh.Scientific reports · 2026Article
- An ArsR-type transcriptional regulator, SoxR, regulates sulfur oxidization (Sox) system in Cupriavidus pinatubonensis JMP134.BMC microbiology · 2026Article
- In Hyphomicrobium denitrificans Two Related Sulfane-Sulfur Responsive Transcriptional Repressors Regulate Thiosulfate Oxidation and Have a Deep Impact on Nitrate Respiration and Anaerobic Biosyntheses.Molecular microbiology · 2025Article
- Oxygen needs sulfur, sulfur needs oxygen: a relationship of interdependence.The EMBO journal · 2025Review
- Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR.Cell death discovery · 2024Article
- Sensing and regulation of reactive sulfur species (RSS) in bacteria.Current opinion in chemical biology · 2023Review
- In the AlphaproteobacteriumAntioxidants (Basel, Switzerland) · 2023Article
- Increased intracellular persulfide levels attenuate HlyU-mediated hemolysin transcriptional activation inbioRxiv : the preprint server for biology · 2023Article
- A sulfide-sensor and a sulfane sulfur-sensor collectively regulate sulfur-oxidation for feather degradation by Bacillus licheniformis.Communications biology · 2023Article
- The Transcriptional Repressor PerR Senses Sulfane Sulfur by Cysteine Persulfidation at the Structural ZnAntioxidants (Basel, Switzerland) · 2023Article
- Generation and Physiology of Hydrogen Sulfide and Reactive Sulfur Species in Bacteria.Antioxidants (Basel, Switzerland) · 2022Review
- The Sulfide-Responsive SqrR/BigR Homologous Regulator YgaV ofAntioxidants (Basel, Switzerland) · 2022Article
- The ArsH Protein Product of theAntioxidants (Basel, Switzerland) · 2022Article
- Structural and mechanistic basis for redox sensing by the cyanobacterial transcription regulator RexT.Communications biology · 2022Article
- Functional asymmetry and chemical reactivity of CsoR family persulfide sensors.Nucleic acids research · 2021Article
- Allopatric Plant Pathogen Population Divergence following Disease Emergence.Applied and environmental microbiology · 2021Article
- Something smells bad to plant pathogens: Production of hydrogen sulfide in plants and its role in plant defence responses.Journal of advanced research · 2021Review
- Structural basis for persulfide-sensing specificity in a transcriptional regulator.Nature chemical biology · 2021Article
- HThe Journal of biological chemistry · 2020Review
- The Response ofmBio · 2020Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Winged-helix transcriptional factors play important roles in the control of gene expression in many organisms. In the plant pathogens Xylella fastidiosa and Agrobacterium tumefaciens, the winged-helix protein BigR, a member of the ArsR/SmtB family of metal sensors, regulates transcription of the bigR operon involved in bacterial biofilm growth. Previous studies showed that BigR represses transcription of its own operon through the occupation of the RNA polymerase-binding site; however, the signals that modulate its activity and the biological function of its operon are still poorly understood. Here we show that although BigR is a homodimer similar to metal sensors, it functions as a novel redox switch that derepresses transcription upon oxidation. Crystal structures of reduced and oxidized BigR reveal that formation of a disulfide bridge involving two critical cysteines induces conformational changes in the dimer that remarkably alter the topography of the winged-helix DNA-binding interface, precluding DNA binding. This structural mechanism of DNA association-dissociation is novel among winged-helix factors. Moreover, we demonstrate that the bigR operon is required for hydrogen sulfide detoxification through the action of a sulfur dioxygenase (Blh) and sulfite exporter. As hydrogen sulfide strongly inhibits cytochrome c oxidase, it must be eliminated to allow aerobic growth under low oxygen tension, an environmental condition found in bacterial biofilms, xylem vessels, and root tissues. Accordingly, we show that the bigR operon is critical to sustain bacterial growth under hypoxia. These results suggest that BigR integrates the transcriptional regulation of a sulfur oxidation pathway to an oxidative signal through a thiol-based redox switch.
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