ArticleMolecular microbiology2011
Vanadate and triclosan synergistically induce alginate production by Pseudomonas aeruginosa strain PAO1.
Article in Molecular microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Enzyme-enhanced RNA isolation from biofilm-producing bacteria.Microbiology spectrum · 2026Article
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- Monoclonal antibodies against lipopolysaccharide protect againstFrontiers in cellular and infection microbiology · 2023Article
- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- Overproduction of the AlgT Sigma Factor Is Lethal to Mucoid Pseudomonas aeruginosa.Journal of bacteriology · 2020Article
- Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis.Nature communications · 2019Article
- Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.Journal of medical microbiology · 2018Article
- The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation inFrontiers in microbiology · 2017Article
- Pseudomonas aeruginosa AmrZ Binds to Four Sites in the algD Promoter, Inducing DNA-AmrZ Complex Formation and Transcriptional Activation.Journal of bacteriology · 2016Article
- The capability of Pseudomonas aeruginosa to recruit zinc under conditions of limited metal availability is affected by inactivation of the ZnuABC transporter.Metallomics : integrated biometal science · 2015Article
- Enzymatic modifications of exopolysaccharides enhance bacterial persistence.Frontiers in microbiology · 2015Review
- LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.Journal of proteomics · 2014Article
- Regulation of bacterial virulence gene expression by cell envelope stress responses.Virulence · 2014Review
- Expression of mucoid induction factor MucE is dependent upon the alternate sigma factor AlgU in Pseudomonas aeruginosa.BMC microbiology · 2013Article
- Genes required for and effects of alginate overproduction induced by growth of Pseudomonas aeruginosa on Pseudomonas isolation agar supplemented with ammonium metavanadate.Journal of bacteriology · 2013Article
- Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.Applied and environmental microbiology · 2013Article
- Identification of the mutation responsible for the temperature-sensitive lipopolysaccharide O-antigen defect in the Pseudomonas aeruginosa cystic fibrosis isolate 2192.Journal of bacteriology · 2013Article
- Evidence for sigma factor competition in the regulation of alginate production by Pseudomonas aeruginosa.PloS one · 2013Article
- Proteolytic regulation of alginate overproduction in Pseudomonas aeruginosa.Molecular microbiology · 2012Review
- Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN.Journal of bacteriology · 2012Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Alginate overproduction by P. aeruginosa strains, also known as mucoidy, is associated with chronic lung infections in cystic fibrosis (CF). It is not clear how alginate induction occurs in the wild-type (wt) mucA strains. When grown on Pseudomonas isolation agar (PIA), P. aeruginosa strains PAO1 and PA14 are non-mucoid, producing minimal amounts of alginate. Here we report the addition of ammonium metavanadate (AMV), a phosphatase inhibitor, to PIA (PIA-AMV) induced mucoidy in both these laboratory strains and early lung colonizing non-mucoid isolates with a wt mucA. This phenotypic switch was reversible depending on the availability of vanadate salts and triclosan, a component of PIA. Alginate induction in PAO1 on PIA-AMV was correlated with increased proteolytic degradation of MucA, and required envelope proteases AlgW or MucP, and a two-component phosphate regulator, PhoP. Other changes included the addition of palmitate to lipid A, a phenotype also observed in chronic CF isolates. Proteomic analysis revealed the upregulation of stress chaperones, which was confirmed by increased expression of the chaperone/protease MucD. Altogether, these findings suggest a model of alginate induction and the PIA-AMV medium may be suitable for examining early lung colonization phenotypes in CF before the selection of the mucA mutants.
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