ArticleMolecular pharmaceutics2011
Duplex end breathing determines serum stability and intracellular potency of siRNA-Au NPs.
Article in Molecular pharmaceutics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Gold Nanoparticles in Conjunction with Nucleic Acids as a Modern Molecular System for Cellular Delivery.Molecules (Basel, Switzerland) · 2020Review
- Attaching DNA to Gold Nanoparticles With a Protein Corona.Frontiers in chemistry · 2020Article
- RNA-Based Immunostimulatory Liposomal Spherical Nucleic Acids as Potent TLR7/8 Modulators.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- MUC1 Aptamer Targeted SERS Nanoprobes.Advanced functional materials · 2017Article
- Controlling DNA-nanoparticle serum interactions.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ribozyme-Spherical Nucleic Acids.Journal of the American Chemical Society · 2015Article
- Spherical nucleic acids as a divergent platform for synthesizing RNA-nanoparticle conjugates through enzymatic ligation.ACS nano · 2014Article
- Knockdown of intraislet IKKβ by spherical nucleic acid conjugates prevents cytokine-induced injury and enhances graft survival.Transplantation · 2013Article
- Targeted delivery of siRNA-generating DNA nanocassettes using multifunctional nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2013Article
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- Delivery of multiple siRNAs using lipid-coated PLGA nanoparticles for treatment of prostate cancer.Nano letters · 2012Article
- Oligonucleotide delivery by cell-penetrating "striped" nanoparticles.Angewandte Chemie (International ed. in English) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Structural requirements of siRNA-functionalized gold nanoparticles (siRNA-Au NPs) for Dicer recognition and serum stability were studied. We show that the 3' overhang on the nucleic acids of these particles is preferentially recognized by Dicer but also makes the siRNA duplexes more susceptible to nonspecific serum degradation. Dicer and serum nucleases show lower preference for blunt duplexes as opposed to those with 3' overhangs. Importantly, gold nanoparticles functionalized with blunt duplexes with relatively less thermal breathing are up to 15 times more stable against serum degradation without compromising Dicer recognition. This increased stability leads to a 300% increase in cellular uptake of siRNA-Au NPs and improved gene knockdown.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.