ArticlePlant cell reports2011
Manipulation of saponin biosynthesis by RNA interference-mediated silencing of β-amyrin synthase gene expression in soybean.
Article in Plant cell reports, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 48 citations in OpenAlex.
- Non-targeted metabolite profiling reveals substantial equivalence of omega-3 enrichedGM crops & food · 2026Article
- A modular UDP-glycosyltransferase and cytochrome P450 system for soyasaponin C-22 modification.The Plant journal : for cell and molecular biology · 2026Article
- Simultaneous site-directed mutagenesis for soybean ß-amyrin synthase genes via DNA-free CRISPR/Cas9 system using a single gRNA.Plant cell reports · 2025Article
- Creating saponin-free yellow pea seeds byPlant direct · 2024Article
- The challenge of breeding for reduced off-flavor in faba bean ingredients.Frontiers in plant science · 2023Review
- Genetic manipulation of anti-nutritional factors in major crops for a sustainable diet in future.Frontiers in plant science · 2022Review
- Oxidosqualene Cyclase Knock-Down in Latex ofMolecules (Basel, Switzerland) · 2019Article
- Article
- Functional analysis of β-amyrin synthase gene in ginsenoside biosynthesis by RNA interference.Plant cell reports · 2015Article
- De novo transcriptome assembly and the putative biosynthetic pathway of steroidal sapogenins of Dioscorea composita.PloS one · 2015Article
- Isolation and characterization of an oxidosqualene cyclase gene encoding a β-amyrin synthase involved in Polygala tenuifolia Willd. saponin biosynthesis.Plant cell reports · 2014Article
- Molecular characterization and differential expression studies of an oxidosqualene cyclase (OSC) gene of Brahmi (Bacopa monniera).Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Article
- Genetic and chemical analysis of a key biosynthetic step for soyasapogenol A, an aglycone of group A saponins that influence soymilk flavor.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Accumulation of β-conglycinin in soybean cotyledon through the formation of disulfide bonds between α'- and α-subunits.Plant physiology · 2012Article
- RNA silencing as a tool to uncover gene function and engineer novel traits in soybean.Breeding science · 2012Article
- Recent advances in soybean transformation and their application to molecular breeding and genomic analysis.Breeding science · 2012Article
- Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soybean seeds contain substantial amount of diverse triterpenoid saponins that influence the seed quality, although little is known about the physiologic functions of saponins in plants. We now describe the modification of saponin biosynthesis by RNA interference (RNAi)-mediated gene silencing targeted to β-amyrin synthase, a key enzyme in the synthesis of a common aglycon of soybean saponins. We identified two putative β-amyrin synthase genes in soybean that manifested distinct expression patterns with regard to developmental stage and tissue specificity. Given that one of these genes, GmBAS1, was expressed at a much higher level than the other (GmBAS2) in various tissues including the developing seeds, we constructed two RNAi vectors that encode self-complementary hairpin RNAs corresponding to the distinct regions of GmBAS1 under the control of a seed-specific promoter derived from the soybean gene for the α' subunit of the seed storage protein β-conglycinin. These vectors were introduced independently into soybean. Six independent transgenic lines exhibited a stable reduction in seed saponin content, with the extent of saponin deficiency correlating with the β-amyrin synthase mRNA depletion. Although some transgenic lines produced seeds almost devoid of saponins, no abnormality in their growth was apparent and the antioxidant activity of their seeds was similar to that of control seeds. These results suggest that saponins are not required for seed development and survival, and that soybean seeds may therefore be amenable to the modification of triterpenoid saponin content and composition through molecular biologic approaches.
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