ArticleProceedings of the National Academy of Sciences of the United States of America2011
Defects in coatomer protein I (COPI) transport cause blood feeding-induced mortality in Yellow Fever mosquitoes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 70 citations in OpenAlex.
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- Mosquito host background impacts microbiome-Zika virus interactions in field- and laboratory-reared Aedes aegypti.Animal microbiome · 2025Article
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- Mosquito gene targeted RNAi studies for vector control.Functional & integrative genomics · 2023Review
- COPB2: a transport protein with multifaceted roles in cancer development and progression.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021Review
- Formoterol PLGA-PEG Nanoparticles Induce Mitochondrial Biogenesis in Renal Proximal Tubules.The AAPS journal · 2021Article
- Activation ofBiomolecules · 2021Article
- Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes.Journal of genomics · 2020Article
- The 5-hydroxytryptamine receptor 1F stimulates mitochondrial biogenesis and angiogenesis in endothelial cells.Biochemical pharmacology · 2019Article
- Identification and characterization of a mosquito-specific eggshell organizing factor in Aedes aegypti mosquitoes.PLoS biology · 2019Article
- Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-CollectedFrontiers in microbiology · 2018Article
- Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis.Scientific reports · 2017Article
- Microbial control of arthropod-borne disease.Memorias do Instituto Oswaldo Cruz · 2017Review
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- RNA interference: a promising biopesticide strategy against the African Sweetpotato Weevil Cylas brunneus.Scientific reports · 2016Article
- Effective disposal of nitrogen waste in blood-fed Aedes aegypti mosquitoes requires alanine aminotransferase.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- In silico models for predicting vector control chemicals targeting Aedes aegypti.SAR and QSAR in environmental research · 2014Review
- COPI-mediated blood meal digestion in vector mosquitoes is independent of midgut ARF-GEF and ARF-GAP regulatory activities.Insect biochemistry and molecular biology · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Blood feeding by vector mosquitoes provides the entry point for disease pathogens and presents an acute metabolic challenge that must be overcome to complete the gonotrophic cycle. Based on recent data showing that coatomer protein I (COPI) vesicle transport is involved in cellular processes beyond Golgi-endoplasmic reticulum retrograde protein trafficking, we disrupted COPI functions in the Yellow Fever mosquito Aedes aegypti to interfere with blood meal digestion. Surprisingly, we found that decreased expression of the γCOPI coatomer protein led to 89% mortality in blood-fed mosquitoes by 72 h postfeeding compared with 0% mortality in control dsRNA-injected blood-fed mosquitoes and 3% mortality in γCOPI dsRNA-injected sugar-fed mosquitoes. Similar results were obtained using dsRNA directed against five other COPI coatomer subunits (α, β, β', δ, and ζ). We also examined midgut tissues by EM, quantitated heme in fecal samples, and characterized feeding-induced protein expression in midgut, fat body, and ovary tissues of COPI-deficient mosquitoes. We found that COPI defects disrupt epithelial cell membrane integrity, stimulate premature blood meal excretion, and block induced expression of several midgut protease genes. To study the role of COPI transport in ovarian development, we injected γCOPI dsRNA after blood feeding and found that, although blood digestion was normal, follicles in these mosquitoes were significantly smaller by 48 h postinjection and lacked eggshell proteins. Together, these data show that COPI functions are critical to mosquito blood digestion and egg maturation, a finding that could also apply to other blood-feeding arthropod vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.