ArticleThe Journal of biological chemistry2011
C termini of proteasomal ATPases play nonequivalent roles in cellular assembly of mammalian 26 S proteasome.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- PI31 is a positive regulator of 20S immunoproteasome assembly.Journal of cell science · 2025Article
- Assembly chaperone Nas6 selectively destabilizes 26S proteasomes with defective regulatory particle-core particle interfaces.The Journal of biological chemistry · 2023Article
- Article
- Structure, Dynamics and Function of the 26S Proteasome.Sub-cellular biochemistry · 2021Review
- Proteasome Activation to Combat Proteotoxicity.Molecules (Basel, Switzerland) · 2019Review
- Aim for the core: suitability of the ubiquitin-independent 20S proteasome as a drug target in neurodegeneration.Translational research : the journal of laboratory and clinical medicine · 2018Review
- Expanded Coverage of the 26S Proteasome Conformational Landscape Reveals Mechanisms of Peptidase Gating.Cell reports · 2018Article
- Phosphatase UBLCP1 controls proteasome assembly.Open biology · 2017Article
- Nucleotide-dependent switch in proteasome assembly mediated by the Nas6 chaperone.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Structural basis for dynamic regulation of the human 26S proteasome.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- ATP binding to neighbouring subunits and intersubunit allosteric coupling underlie proteasomal ATPase function.Nature communications · 2015Article
- Proteasome Activation is Mediated via a Functional Switch of the Rpt6 C-terminal Tail Following Chaperone-dependent Assembly.Scientific reports · 2015Article
- Dysfunction in protein clearance by the proteasome: impact on autoinflammatory diseases.Seminars in immunopathology · 2015Review
- N-Terminal Coiled-Coil Structure of ATPase Subunits of 26S Proteasome Is Crucial for Proteasome Function.PloS one · 2015Article
- Molecular and cellular roles of PI31 (PSMF1) protein in regulation of proteasome function.The Journal of biological chemistry · 2014Article
- A chaperone-assisted degradation pathway targets kinetochore proteins to ensure genome stability.PLoS genetics · 2014Article
- Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase.Nature structural & molecular biology · 2013Article
- Article
- ATP binding by proteasomal ATPases regulates cellular assembly and substrate-induced functions of the 26 S proteasome.The Journal of biological chemistry · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The 26 S proteasome comprises two multisubunit subcomplexes as follows: 20 S proteasome and PA700/19 S regulatory particle. The cellular mechanisms by which these subcomplexes assemble into 26 S proteasome and the molecular determinants that govern the assembly process are poorly defined. Here, we demonstrate the nonequivalent roles of the C termini of six AAA subunits (Rpt1-Rpt6) of PA700 in 26 S proteasome assembly in mammalian cells. The C-terminal HbYX motif (where Hb is a hydrophobic residue, Y is tyrosine, and X is any amino acid) of each of two subunits, Rpt3 and Rpt5, but not that of a third subunit Rpt2, was essential for assembly of 26 S proteasome. The C termini of none of the three non-HbYX motif Rpt subunits were essential for cellular 26 S proteasome assembly, although deletion of the last three residues of Rpt6 destabilized the 20 S-PA700 interaction. Rpt subunits defective for assembly into 26 S proteasome due to C-terminal truncations were incorporated into intact PA700. Moreover, intact PA700 accumulated as an isolated subcomplex when cellular 20 S proteasome content was reduced by RNAi. These results indicate that 20 S proteasome is not an obligatory template for assembly of PA700. Collectively, these results identify specific structural elements of two Rpt subunits required for 26 S proteasome assembly, demonstrate that PA700 can be assembled independently of the 20 S proteasome, and suggest that intact PA700 is a direct intermediate in the cellular pathway of 26 S proteasome assembly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.