ArticleCytotechnology2011
Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.
Article in Cytotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed.
- Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.Molecular nutrition & food research · 2026Article
- Investigating alterations associated with heat stress and the recovery of the intestinal barrier using IPEC-J2 as an intestinal epithelial porcine cell model.Scientific reports · 2026Article
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Escherichia coli HPI-induced duodenitis through ubiquitin regulation of the TLR4/NF-κB pathway.BMC veterinary research · 2025Article
- Article
- New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells.BMC research notes · 2024Article
- Immunomodulatory effect of probiotic exopolysaccharides in a porcine in vitro co-culture model mimicking the intestinal environment on ETEC infection.Veterinary research communications · 2024Article
- Isolation and Identification of a Tibetan Pig Porcine Epidemic Diarrhoea Virus Strain and Its Biological Effects on IPEC-J2 Cells.International journal of molecular sciences · 2024Article
- Article
- Development of an intestinal epithelial cell line and organoids derived from the same swine and characterization of their antiviral responses.Bioscience of microbiota, food and health · 2024Article
- Drinking alkaline mineral water confers diarrhea resistance in maternally separated piglets by maintaining intestinal epithelial regeneration via the brain-microbe-gut axis.Journal of advanced research · 2023Article
- Air-liquid interface cultures trigger a metabolic shift in intestinal epithelial cells (IPEC-1).Histochemistry and cell biology · 2023Article
- Conditionally immortalised equine skeletal muscle cell lines forBiochemistry and biophysics reports · 2023Article
- In vitro investigation of monoglycerides and zinc glycinate: anti-inflammatory and epithelial barrier function.Journal of animal science · 2023Article
- Different Mechanisms Are Utilized by Coronavirus Transmissible Gastroenteritis Virus To Regulate Interferon Lambda 1 and Interferon Lambda 3 Production.Journal of virology · 2022Article
- Effect of probiotic bacteria on porcine rotavirus OSU infection of porcine intestinal epithelial IPEC-J2 cells.Archives of virology · 2022Article
- Comparison of expression profiles between undifferentiated and differentiated porcine IPEC-J2 cells.Porcine health management · 2022Article
- Identification of mutations in porcineFrontiers in veterinary science · 2022Article
- Relationship between serum concentration, functional parameters and cell bioenergetics in IPEC-J2 cell line.Histochemistry and cell biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high similarity between pigs and humans makes pigs a good gastrointestinal (GI) model for humans. Recently an epithelial cell line originating from the jejunum of pig (IPEC-J2) became available. Once validated, this model can be used to investigate the complex interactions occurring in the intestine. The advantages of using IPEC-J2 as in vitro model of the GI tract are the high resemblance between humans and pigs, and the ease of extrapolating in vitro to in vivo characteristics. In this study, the IPEC-J2 cells were functionally characterized by measuring the trans-epithelial electrical resistance (TEER), and by histological and ultrastructural studies. IPEC-J2 cells grown on six different permeable support systems, were investigated. The Transwell(®)-COL collagen-coated membrane (1.12 cm(2)) showed the best results concerning time efficiency and TEER values. The optimum seeding density of 12 × 10(5) cells/mL ensured that after 9 days of differentiation a confluent monolayer was formed. The decrease in TEER values after a maximum had been reached, coincided with the ultrastructural development of apical microvilli. We conclude that IPEC-J2 cells grown on collagen-coated membranes represent a valuable in vitro model system for the small intestinal epithelium which can be of great interest for intestinal research.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.