ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2011
Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 24 citations in OpenAlex.
- Gapless and phased genome assembly reveals post-allopolyploidization subgenomic diversification of tobacco centromeres.Genome biology · 2026Article
- Pancentromere analysis of Allium species reveals diverse centromere positions in onion and gigantic centromeres in garlic.The Plant cell · 2025Article
- The genome and GeneBank genomics of allotetraploid Nicotiana tabacum provide insights into genome evolution and complex trait regulation.Nature genetics · 2025Article
- Chromosome-specific barcode system with centromeric repeat in cultivated soybean and wild progenitor.Life science alliance · 2024Article
- The complete genome assembly of Nicotiana benthamiana reveals the genetic and epigenetic landscape of centromeres.Nature plants · 2024Article
- Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids.Frontiers in plant science · 2024Article
- Moonlight Is Perceived as a Signal Promoting Genome Reorganization, Changes in Protein and Metabolite Profiles and Plant Growth.Plants (Basel, Switzerland) · 2023Article
- Article
- Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat.Frontiers in plant science · 2015Article
- Chromosomal organizations of major repeat families on potato (Solanum tuberosum) and further exploring in its sequenced genome.Molecular genetics and genomics : MGG · 2014Article
- Tobacco karyotyping by accurate centromere identification and novel repetitive DNA localization.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- Isolation of centromeric-tandem repetitive DNA sequences by chromatin affinity purification using a HaloTag7-fused centromere-specific histone H3 in tobacco.Plant cell reports · 2012Article
- Computational and experimental analyses of retrotransposon-associated minisatellite DNAs in the soybean genome.BMC bioinformatics · 2012Article
- Chromosome dynamics visualized with an anti-centromeric histone H3 antibody in Allium.PloS one · 2012Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although a centromeric DNA fragment of tobacco (Nicotiana tabacum), Nt2-7, has been reported, the overall structure of the centromeres remains unknown. To characterize the centromeric DNA sequences, we conducted a chromatin immunoprecipitation assay using anti-NtCENH3 antibody and chromatins isolated from two ancestral diploid species (Nicotiana sylvestris and Nicotiana tomentosiformis) of N. tabacum and isolated a 178-pb fragment, Nto1 from N. tomentosiformis, as a novel centromeric DNA. Fluorescence in situ hybridization (FISH) showed that Nto1 localizes on 24 out of 48 chromosomes in some cells of a BY-2 cell line. To identify the origins of the Nt2-7 and Nto1, a tobacco bacterial artificial chromosome (BAC) library was constructed from N. tabacum, and then screened by polymerase chain reaction (PCR) with primer sets designed from the Nt2-7 and Not1 DNA sequences. Twelve BAC clones were found to localize on the centromeric regions by FISH. We selected three BAC clones for sequencing and identified two centromeric retrotransposons, NtCR and NtoCR, the DNA sequences of which are similar to that of Nt2-7 and Nto1, respectively. Quantitative PCR analysis using coprecipitated DNA with anti-NtCENH3 clearly showed coexistence of NtCENH3 with both retrotransposons. These results indicate the possibility that these two retrotransposons act as centromeric DNA sequences in tobacco. NtoCR was found to be specific to N. tomentosiformis and T genome of N. tabacum, and a NtCR-like centromeric retrotransposon (TGRIV) exists in tomato. This specificity suggests that the times of amplification of these centromeric retrotransposons were different.
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