ArticleInternational journal of legal medicine2012
Potential forensic application of DNA methylation profiling to body fluid identification.
Article in International journal of legal medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 149 citations in OpenAlex.
- Multiplex methylation-specific PCR for distinguishing semen, saliva, and blood.International journal of legal medicine · 2026Article
- Recent advances in the identification of vaginal fluid for forensic applications.International journal of legal medicine · 2026Review
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- High resolution tissue and cell type identification via single cell transcriptomic profiling.PloS one · 2025Article
- Application of a new composite genetic marker semen-specific methylation-microhaplotype in the analysis of semen-vaginal fluid mixtures.Royal Society open science · 2025Article
- Development of a novel panel for blood identification based on blood-specific CpG-linked SNP markers.International journal of legal medicine · 2024Article
- Identification of Mixtures of Two Types of Body Fluids Using the Multiplex Methylation System and Random Forest Models.Current medical science · 2023Article
- Selection and validation of a novel set of specific differential methylation markers and construction of a random forest prediction model for the accurate tissue origin identifications of body fluids involving young and middle-aged group of Chinese Han population.International journal of legal medicine · 2023Article
- scHiMe: predicting single-cell DNA methylation levels based on single-cell Hi-C data.Briefings in bioinformatics · 2023Article
- Establishment of a co-analysis system for personal identification and body fluid identification: a preliminary report.International journal of legal medicine · 2022Article
- Article
- Current Methods for Body Fluid Identification Related to Sexual Crime: Focusing on Saliva, Semen, and Vaginal Fluid.Diagnostics (Basel, Switzerland) · 2020Review
- Characterization of DNA methylation-based markers for human body fluid identification in forensics: a critical review.International journal of legal medicine · 2020Review
- Specific microbes of saliva and vaginal fluid of Guangdong Han females based on 16S rDNA high-throughput sequencing.International journal of legal medicine · 2019Article
- Marker evaluation for differentiation of blood and menstrual fluid by methylation-sensitive SNaPshot analysis.International journal of legal medicine · 2018Article
- Article
- High resolution melt curve analysis based on methylation status for human semen identification.Forensic science, medicine, and pathology · 2017Article
- Forensic DNA methylation profiling from evidence material for investigative leads.BMB reports · 2016Review
- Novel identification of biofluids using a multiplex methylation-specific PCR combined with single-base extension system.Forensic science, medicine, and pathology · 2016Article
- Methylation Markers for the Identification of Body Fluids and Tissues from Forensic Trace Evidence.PloS one · 2016Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called tDMRs (tissue-specific differentially methylated regions) show different DNA methylation profiles according to the type of cell or tissue. We examined the potential of tissue-specific differential DNA methylation for body fluid identification. Five tDMRs for the genes DACT1, USP49, HOXA4, PFN3, and PRMT2 were selected, and DNA methylation profiles for these tDMRs were produced by bisulfite sequencing using pooled DNA from blood, saliva, semen, menstrual blood, and vaginal fluid. The tDMRs for DACT1 and USP49 showed semen-specific hypomethylation, and the tDMRs for HOXA4, PFN3, and PRMT2 displayed varying degrees of methylation according to the type of body fluid. Preliminary tests using methylation-specific PCR for the DACT1 and USP49 tDMRs showed that these two markers could be used successfully to identify semen samples including sperm cells. Body fluid-specific differential DNA methylation may be a promising indicator for body fluid identification. Because DNA methylation profiling uses the same biological source of DNA for individual identification profiling, the determination of more body fluid-specific tDMRs and the development of convenient tDMR analysis methods will facilitate the broad implementation of body fluid identification in forensic casework.
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