Evidence map›Paper›PMID 21625864›Full record

ArticleMolecular biology reports2012

Forensic species identification of large macaws using DNA barcodes and microsatellite profiles.

Hideaki Abe, Azusa Hayano, Miho Inoue-Murayama

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Hideaki AbeWildlife Research Center of Kyoto University, 2-24 Tanaka-Sekiden-cho, Sakyo-ku, Kyoto, 606-8203, Japan.
Azusa Hayano
Miho Inoue-Murayama

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using mitochondrial and nuclear markers species identification was conducted in the case of seized feathers. Earlier, we had sequenced cytochrome c oxidase subunit I (COI) both from 10 seized specimens and 43 validation specimens from captive macaws belonging to 4 Ara species (A. macao, A. chloropterus, A. ararauna, and A. ambiguus) and identified 19 haplotypes based on COI sequences. Species-level identification using Barcode of Life Data Systems showed that seized feathers shared the highest similarity with scarlet macaws (A. macao), and this result was supported by the tree-base identification with high bootstrap values. Moreover, microsatellite profiles in AgGT17 locus showed that patterns of allelic distribution in the seized feathers were apparently distinct from those of red-and-green macaw (A. chloropterus), but were overlapped with those of A. macao, suggesting that all of seized feathers were derived from several individuals of A. macao. We also determined the parentage of hybrid macaws by the combination of COI barcodes and microsatellite profiles. The technique presented here will contribute to forensic identification and future conservation of large macaws that have been lost due to deforestation.

Indexed as

DNA Barcoding, TaxonomicAnimalsBase SequenceCluster AnalysisConservation of Natural ResourcesDNA PrimersFeathersFemaleHaplotypesMaleMicrosatellite RepeatsModels, GeneticMolecular Sequence DataPsittaciformesSequence Analysis, DNASpecies SpecificityDNA Primers

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.