ArticlePLoS pathogens2011
Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Managing Plant Viruses in Tissue-Cultured Apple and Grapevine: Strategies for Detection and Eradication.The plant pathology journal · 2025Review
- Predictive Modeling of Proteins Encoded by a Plant Virus Sheds a New Light on Their Structure and Inherent Multifunctionality.Biomolecules · 2024Article
- Efficiency and Persistence of MoventoPlants (Basel, Switzerland) · 2021Article
- Detection and diversity of viruses infecting African yam (Plant pathology · 2021Article
- Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release.Communications biology · 2020Article
- Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- From a Movement-Deficient Grapevine Fanleaf Virus to the Identification of a New Viral Determinant of Nematode Transmission.Viruses · 2019Article
- Article
- Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein.BMC biotechnology · 2019Article
- In Vitro Acquisition of Specific Small Interfering RNAs Inhibits the Expression of Some Target Genes in the Plant EctoparasiteInternational journal of molecular sciences · 2019Article
- Production and Partial Purification of theIranian journal of biotechnology · 2018Article
- Nanobody-mediated resistance to Grapevine fanleaf virus in plants.Plant biotechnology journal · 2018Article
- Efficient Detection of Long dsRNAFrontiers in plant science · 2018Article
- Correction: Structural Insights into Viral Determinants of Nematode Mediated Grapevine fanleaf virus Transmission.PLoS pathogens · 2017Article
- Article
- Display of whole proteins on inner and outer surfaces of grapevine fanleaf virus-like particles.Plant biotechnology journal · 2016Article
- Biological and molecular events associated with simultaneous transmission of plant viruses by invertebrate and fungal vectors.Molecular plant pathology · 2014Article
- Top 10 plant-parasitic nematodes in molecular plant pathology.Molecular plant pathology · 2013Review
- Complete nucleotide sequences and genome organization of a cherry isolate of cherry leaf roll virus.Archives of virology · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like virus from plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists of 60 identical coat protein subunits (CP), carries the determinants of this specificity. Here, we provide novel insight into GFLV transmission by nematodes through a comparative structural and functional analysis of two GFLV variants. We isolated a mutant GFLV strain (GFLV-TD) poorly transmissible by nematodes, and showed that the transmission defect is due to a glycine to aspartate mutation at position 297 (Gly297Asp) in the CP. We next determined the crystal structures of the wild-type GFLV strain F13 at 3.0 Å and of GFLV-TD at 2.7 Å resolution. The Gly297Asp mutation mapped to an exposed loop at the outer surface of the capsid and did not affect the conformation of the assembled capsid, nor of individual CP molecules. The loop is part of a positively charged pocket that includes a previously identified determinant of transmission. We propose that this pocket is a ligand-binding site with essential function in GFLV transmission by X. index. Our data suggest that perturbation of the electrostatic landscape of this pocket affects the interaction of the virion with specific receptors of the nematode's feeding apparatus, and thereby severely diminishes its transmission efficiency. These data provide a first structural insight into the interactions between a plant virus and a nematode vector.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.