ArticlePloS one2011
Large-scale analysis of acute ethanol exposure in zebrafish development: a critical time window and resilience.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Contributions of Zebrafish Studies to the Behavioural Consequences of Early Alcohol Exposure: A Systematic Review.Current neuropharmacology · 2022Pooled it
- Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.Journal of biomedical optics · 2026Article
- The Role of the Hedgehog Pathway in Alcohol-Induced Birth Defects.Advances in experimental medicine and biology · 2026Review
- Food-induced phenotypic plasticity in echinoid larvae is inducible and reversible throughout development.BMC biology · 2025Article
- Behavioral Profiling of Zebrafish (Danio rerio) Larvae: Activity, Anxiety, Avoidance, and Startle Response.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- Review
- Neurospecific fabrication and toxicity assessment of a PNIPAM nanogel encapsulated withHeliyon · 2022Article
- An integrated systems-level model of ochratoxin A toxicity in the zebrafish (Danio rerio) embryo based on NMR metabolic profiling.Scientific reports · 2022Article
- Article
- Rrn3 gene knockout affects ethanol-induced locomotion in adult heterozygous zebrafish.Psychopharmacology · 2022Article
- Zebrafish models of fetal alcohol spectrum disorders.Genesis (New York, N.Y. : 2000) · 2021Review
- A millifluidic chip for cultivation of fish embryos and toxicity testing fabricated by 3D printing technology.RSC advances · 2021Article
- Zebrafish as a Model for Fetal Alcohol Spectrum Disorders.Frontiers in pharmacology · 2021Review
- Corrigendum: Zebrafish Models of Craniofacial Malformations: Interactions of Environmental Factors.Frontiers in cell and developmental biology · 2021Article
- Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD).Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- Ethanol and Cannabinoids Regulate Zebrafish GABAergic Neuron Development and Behavior in a Sonic Hedgehog and Fibroblast Growth Factor-Dependent Mechanism.Alcoholism, clinical and experimental research · 2020Article
- Animal models of gene-alcohol interactions.Birth defects research · 2020Review
- Zebrafish Shoaling, Its Behavioral and Neurobiological Mechanisms, and Its Alteration by Embryonic Alcohol Exposure: A Review.Frontiers in behavioral neuroscience · 2020Review
- Zebrafish Models of Craniofacial Malformations: Interactions of Environmental Factors.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn humans, ethanol exposure during pregnancy causes a spectrum of developmental defects (fetal alcohol syndrome or FAS). Individuals vary in phenotypic expression. Zebrafish embryos develop FAS-like features after ethanol exposure. In this study, we ask whether stage-specific effects of ethanol can be identified in the zebrafish, and if so, whether they allow the pinpointing of sensitive developmental mechanisms. We have therefore conducted the first large-scale (>1500 embryos) analysis of acute, stage-specific drug effects on zebrafish development, with a large panel of readouts. METHODOLOGY/PRINCIPAL
findingsZebrafish embryos were raised in 96-well plates. Range-finding indicated that 10% ethanol for 1 h was suitable for an acute exposure regime. High-resolution magic-angle spinning proton magnetic resonance spectroscopy showed that this produced a transient pulse of 0.86% concentration of ethanol in the embryo within the chorion. Survivors at 5 days postfertilisation were analysed. Phenotypes ranged from normal (resilient) to severely malformed. Ethanol exposure at early stages caused high mortality (≥88%). At later stages of exposure, mortality declined and malformations developed. Pharyngeal arch hypoplasia and behavioral impairment were most common after prim-6 and prim-16 exposure. By contrast, microphthalmia and growth retardation were stage-independent.
conclusionsOur findings show that some ethanol effects are strongly stage-dependent. The phenotypes mimic key aspects of FAS including craniofacial abnormality, microphthalmia, growth retardation and behavioral impairment. We also identify a critical time window (prim-6 and prim-16) for ethanol sensitivity. Finally, our identification of a wide phenotypic spectrum is reminiscent of human FAS, and may provide a useful model for studying disease resilience.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.