ArticlePloS one2011
Visual genome-wide RNAi screening to identify human host factors required for Trypanosoma cruzi infection.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Localized delivery of coding nucleic acids into adherent cells by in situ electroporation: integrated impedance-based monitoring allows for loss-of-function or gain-of-function screening.Scientific reports · 2026Article
- Impact of Laboratory-Adapted IntracellularMicroorganisms · 2023Article
- Past and future of trypanosomatids high-throughput phenotypic screening.Memorias do Instituto Oswaldo Cruz · 2022Article
- Functional annotation of lncRNA in high-throughput screening.Essays in biochemistry · 2021Review
- Drug Discovery for Chagas Disease: Impact of Different Host Cell Lines on Assay Performance and Hit Compound Selection.Tropical medicine and infectious disease · 2019Article
- CRISPR/Cas9-based gene targeting using synthetic guide RNAs enables robust cell biological analyses.Molecular biology of the cell · 2018Article
- Smooth 2D manifold extraction from 3D image stack.Nature communications · 2017Article
- Non-coding RNAs in Host-Pathogen Interactions: Subversion of Mammalian Cell Functions by Protozoan Parasites.Frontiers in microbiology · 2017Review
- Contribution of high-content imaging technologies to the development of anti-infective drugs.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016Review
- Prokineticin receptor identified by phage display is an entry receptor for Trypanosoma cruzi into mammalian cells.Parasitology research · 2015Article
- Nitroheterocyclic compounds are more efficacious than CYP51 inhibitors against Trypanosoma cruzi: implications for Chagas disease drug discovery and development.Scientific reports · 2014Article
- Phenotypic MicroRNA Microarrays.Microarrays (Basel, Switzerland) · 2013Article
- siRNA Genome Screening Approaches to Therapeutic Drug Repositioning.Pharmaceuticals (Basel, Switzerland) · 2013Article
- Host metabolism regulates intracellular growth of Trypanosoma cruzi.Cell host & microbe · 2013Article
- A genome-wide siRNA screen to identify host factors necessary for growth of the parasite Toxoplasma gondii.PloS one · 2013Article
- Article
- Host gene targets for novel influenza therapies elucidated by high-throughput RNA interference screens.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Review
- RNAi screening: new approaches, understandings, and organisms.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
The protozoan parasite Trypanosoma cruzi is the etiologic agent of Chagas disease, a neglected tropical infection that affects millions of people in the Americas. Current chemotherapy relies on only two drugs that have limited efficacy and considerable side effects. Therefore, the development of new and more effective drugs is of paramount importance. Although some host cellular factors that play a role in T. cruzi infection have been uncovered, the molecular requirements for intracellular parasite growth and persistence are still not well understood. To further study these host-parasite interactions and identify human host factors required for T. cruzi infection, we performed a genome-wide RNAi screen using cellular microarrays of a printed siRNA library that spanned the whole human genome. The screening was reproduced 6 times and a customized algorithm was used to select as hits those genes whose silencing visually impaired parasite infection. The 162 strongest hits were subjected to a secondary screening and subsequently validated in two different cell lines. Among the fourteen hits confirmed, we recognized some cellular membrane proteins that might function as cell receptors for parasite entry and others that may be related to calcium release triggered by parasites during cell invasion. In addition, two of the hits are related to the TGF-beta signaling pathway, whose inhibition is already known to diminish levels of T. cruzi infection. This study represents a significant step toward unveiling the key molecular requirements for host cell invasion and revealing new potential targets for antiparasitic therapy.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.