ArticlePloS one2011
Unfaithful maintenance of methylation imprints due to loss of maternal nuclear Dnmt1 during somatic cell nuclear transfer.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- 5-Methylcytosine Methylation-Linked Hippo Pathway Molecular Interactions Regulate Lipid Metabolism.International journal of molecular sciences · 2025Review
- Dysregulated Gene Expression of Imprinted and X-Linked Genes: A Link to Poor Development of Bovine Haploid Androgenetic Embryos.Frontiers in cell and developmental biology · 2021Article
- Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.International journal of molecular sciences · 2020Review
- Generation of Transgenic Cloned Buffalo Embryos Harboring the EGFP Gene in the Y Chromosome Using CRISPR/Cas9-Mediated Targeted Integration.Frontiers in veterinary science · 2020Article
- Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos.The Journal of reproduction and development · 2016Article
- Epigenetic Modification of Cloned Embryos Improves Nanog Reprogramming in Pigs.Cellular reprogramming · 2015Article
- Enriched environment-induced maternal weight loss reprograms metabolic gene expression in mouse offspring.The Journal of biological chemistry · 2015Article
- Trichostatin A rescues the disrupted imprinting induced by somatic cell nuclear transfer in pigs.PloS one · 2015Article
- A novel role for DNA methyltransferase 1 in regulating oocyte cytoplasmic maturation in pigs.PloS one · 2015Article
- Epigenetic Modification Agents Improve Gene-Specific Methylation Reprogramming in Porcine Cloned Embryos.PloS one · 2015Article
- Aberrant expression of Xist in aborted porcine fetuses derived from somatic cell nuclear transfer embryos.International journal of molecular sciences · 2014Article
- Paternally induced transgenerational inheritance of susceptibility to diabetes in mammals.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Epigenetic modification agents improve genomic methylation reprogramming in porcine cloned embryos.The Journal of reproduction and development · 2014Article
- Generation and developmental characteristics of porcine tetraploid embryos and tetraploid/diploid chimeric embryos.Genomics, proteomics & bioinformatics · 2013Article
- Treating cloned embryos, but not donor cells, with 5-aza-2'-deoxycytidine enhances the developmental competence of porcine cloned embryos.The Journal of reproduction and development · 2013Article
- Effects of DNMT1 and HDAC inhibitors on gene-specific methylation reprogramming during porcine somatic cell nuclear transfer.PloS one · 2013Article
- Differential differences in methylation status of putative imprinted genes among cloned swine genomes.PloS one · 2012Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The low success rate of somatic cell nuclear transfer (SCNT) in mammalian cloning is largely due to imprinting problems. However, little is known about the mechanisms of reprogramming imprinted genes during SCNT. Parental origin-specific DNA methylation regulates the monoallelic expression of imprinted genes. In natural fertilization, methylation imprints are established in the parental germline and maintained throughout embryonic development. However, it is unclear whether methylation imprints are protected from global changes of DNA methylation in cloned preimplantation embryos. Here, we demonstrate that cloned porcine preimplantation embryos exhibit demethylation at differentially methylated regions (DMRs) of imprinted genes; in particular, demethylation occurs during the first two cell cycles. By RNAi-mediated knockdown, we found that Dnmt1 is required for the maintenance of methylation imprints in porcine preimplantation embryos. However, no clear signals were detected in the nuclei of oocytes and preimplantation embryos by immunofluorescence. Thus, Dnmt1 is present at very low levels in the nuclei of porcine oocytes and preimplantation embryos and maintains methylation imprints. We further showed that methylation imprints were rescued in nonenucleated metaphase II (MII) oocytes. Our results indicate that loss of Dnmt1 in the maternal nucleus during SCNT significantly contributes to the unfaithful maintenance of methylation imprints in cloned embryos.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.